ArticleApplied and environmental microbiology2012
Detection and differentiation of avian mycoplasmas by surface-enhanced Raman spectroscopy based on a silver nanorod array.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Serological evidence and risk factors ofOpen veterinary journal · 2025Article
- Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of EgyptianPathogens (Basel, Switzerland) · 2023Article
- Article
- Genome Editing of Veterinary Relevant Mycoplasmas Using a CRISPR-Cas Base Editor System.Applied and environmental microbiology · 2022Article
- First Molecular Survey to DetectAnimals : an open access journal from MDPI · 2022Article
- Seroprevalence and Associated Risk Factors ofVeterinary medicine (Auckland, N.Z.) · 2022Article
- Unraveling the Global Phylodynamic and Phylogeographic Expansion ofPathogens (Basel, Switzerland) · 2020Article
- Optimization of a hybrid plasmonic configuration: particle on a corrugated film and its SERS application.RSC advances · 2019Article
- Bird feathers as potential sources of pathogenic microorganisms: a new look at old diseases.Antonie van Leeuwenhoek · 2018Review
- VARIABLE SELECTION AND BIOMARKER CORRELATION IN THE ANALYSIS OFAnalytical letters · 2017Article
- Serological Screening Suggests Extensive Presence ofJournal of veterinary medicine · 2017Article
- Detection of neuraminidase stalk motifs associated with enhanced N1 subtype influenza A virulence via Raman spectroscopy.The Analyst · 2015Article
- Layer-by-layer polyelectrolyte encapsulation of Mycoplasma pneumoniae for enhanced Raman detection.The Analyst · 2014Article
- Identification of virulence determinants in influenza viruses.Analytical chemistry · 2014Article
- Ag nanorod based surface-enhanced Raman spectroscopy applied to bioanalytical sensing.Journal of biophotonics · 2013Review
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Authors and funding
7 authors.
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Abstract
Mycoplasma gallisepticum is a bacterial pathogen of poultry that is estimated to cause annual losses exceeding $780 million. The National Poultry Improvement Plan guidelines recommend regular surveillance and intervention strategies to contain M. gallisepticum infections and ensure mycoplasma-free avian stocks, but several factors make detection of M. gallisepticum and diagnosis of M. gallisepticum infection a major challenge. Current techniques are laborious, require special expertise, and are typically plagued by false results. In this study, we describe a novel detection strategy which uses silver nanorod array-surface-enhanced Raman spectroscopy (NA-SERS) for direct detection of avian mycoplasmas. As a proof of concept for use in avian diagnostics, we used NA-SERS to detect and differentiate multiple strains of avian mycoplasma species, including Acholeplasma laidlawii, Mycoplasma gallinarum, Mycoplasma gallinaceum, Mycoplasma synoviae, and M. gallisepticum, including vaccine strains 6/85, F, and ts-11. Chemometric multivariate analysis of spectral data was used to classify these species rapidly and accurately, with >93% sensitivity and specificity. Furthermore, NA-SERS had a lower limit of detection that was 100-fold greater than that of standard PCR and comparable to that of real-time quantitative PCR. Detection of M. gallisepticum in choanal cleft swabs from experimentally infected birds yielded good sensitivity and specificity, suggesting that NA-SERS is applicable for clinical detection.
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