ArticleApplied and environmental microbiology2012
Enhanced microbial utilization of recalcitrant cellulose by an ex vivo cellulosome-microbe complex.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 74 citations in OpenAlex.
- Structural and functional insights into cellulosomes: masters of plant cell wall degradation.Frontiers in microbiology · 2025Review
- Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries.Scientific reports · 2024Article
- Sorption of Cellulases in Biofilm Enhances Cellulose Degradation byMicroorganisms · 2022Article
- Bottom-up synthetic ecology study of microbial consortia to enhance lignocellulose bioconversion.Biotechnology for biofuels and bioproducts · 2022Review
- Editorial: Microorganisms for Consolidated 2nd Generation Biorefining.Frontiers in microbiology · 2022Article
- Recent progress on bio-succinic acid production from lignocellulosic biomass.World journal of microbiology & biotechnology · 2021Review
- Cellulosomes: Highly Efficient Cellulolytic Complexes.Sub-cellular biochemistry · 2021Review
- Substrate-Related Factors Affecting Cellulosome-Induced Hydrolysis for Lignocellulose Valorization.International journal of molecular sciences · 2019Review
- Elucidating structure-performance relationships in whole-cell cooperative enzyme catalysis.Nature catalysis · 2019Article
- Stabilizing displayed proteins on vegetative Bacillus subtilis cells.Applied microbiology and biotechnology · 2018Article
- Assembly of Synthetic Functional Cellulosomal Structures onto the Cell Surface of Lactobacillus plantarum, a Potent Member of the Gut Microbiome.Applied and environmental microbiology · 2018Article
- Intein-mediated assembly of tunable scaffoldins for facile synthesis of designer cellulosomes.Applied microbiology and biotechnology · 2018Article
- Biomimetic strategy for constructingBiotechnology for biofuels · 2018Article
- Profiling of Saccharomyces cerevisiae transcription factors for engineering the resistance of yeast to lignocellulose-derived inhibitors in biomass conversion.Microbial cell factories · 2017Article
- Article
- Functional Gene Diversity and Metabolic Potential of the Microbial Community in an Estuary-Shelf Environment.Frontiers in microbiology · 2017Article
- Design of nanoscale enzyme complexes based on various scaffolding materials for biomass conversion and immobilization.Biotechnology journal · 2016Review
- Proof of concept for the simplified breakdown of cellulose by combining Pseudomonas putida strains with surface displayed thermophilic endocellulase, exocellulase and β-glucosidase.Microbial cell factories · 2016Article
- Fuelling the future: microbial engineering for the production of sustainable biofuels.Nature reviews. Microbiology · 2016Review
- Engineering Novel and Improved Biocatalysts by Cell Surface Display.Industrial & engineering chemistry research · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cellulosome-microbe complex was assembled ex vivo on the surface of Bacillus subtilis displaying a miniscaffoldin that can bind with three dockerin-containing cellulase components: the endoglucanase Cel5, the processive endoglucanase Cel9, and the cellobiohydrolase Cel48. The hydrolysis performances of the synthetic cellulosome bound to living cells, the synthetic cellulosome, a noncomplexed cellulase mixture with the same catalytic components, and a commercial fungal enzyme mixture were investigated on low-accessibility recalcitrant Avicel and high-accessibility regenerated amorphous cellulose (RAC). The cell-bound cellulosome exhibited 4.5- and 2.3-fold-higher hydrolysis ability than cell-free cellulosome on Avicel and RAC, respectively. The cellulosome-microbe synergy was not completely explained by the removal of hydrolysis products from the bulk fermentation broth by free-living cells and appeared to be due to substrate channeling of long-chain hydrolysis products assimilated by the adjacent cells located in the boundary layer. Our results implied that long-chain hydrolysis products in the boundary layer may inhibit cellulosome activity to a greater extent than the short-chain products in bulk phase. The findings that cell-bound cellulosome expedited the microbial cellulose utilization rate by 2.3- to 4.5-fold would help in the development of better consolidated bioprocessing microorganisms (e.g., B. subtilis) that can hydrolyze recalcitrant cellulose rapidly at low secretory cellulase levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.