ArticleGenetics2012
A novel approach for directing transgene expression in Drosophila: T2A-Gal4 in-frame fusion.
Article in Genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
What it found
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Who cites it
83 citing papers in PubMed, 134 citations in OpenAlex.
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- Functional profiling and visualization of the sphingolipid metabolic network in vivo.EMBO reports · 2025Article
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- Gal4 drivers of the geosmin receptor Or56a exhibit ectopic expression in the labral sense organ of Drosophila.Scientific reports · 2025Article
- Optimized genetic tools for neuroanatomical and functional mapping of the Aedes aegypti olfactory system.G3 (Bethesda, Md.) · 2025Article
- LGR signaling mediates muscle-adipose tissue crosstalk and protects against diet-induced insulin resistance.Nature communications · 2024Article
- Hox gene-specific cellular targeting using split intein Trojan exons.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Eclosion muscles secrete ecdysteroids to initiate asymmetric intestinal stem cell division in Drosophila.Developmental cell · 2024Article
- Catalytic activity of the Bin3/MePCE methyltransferase domain is dispensable for 7SK snRNP function in Drosophila melanogaster.Genetics · 2024Article
- In vivo activities of heparan sulfate differentially modified by NDSTs during development.Proteoglycan research · 2024Article
- SynLight: a bicistronic strategy for simultaneous active zone and cell labeling in the Drosophila nervous system.G3 (Bethesda, Md.) · 2023Article
- Evidence that tissue recoil in the earlyMolecular biology of the cell · 2023Article
- Using single-cell RNA sequencing to generate predictive cell-type-specific split-GAL4 reagents throughout development.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- SynLight: a dicistronic strategy for simultaneous active zone and cell labeling in thebioRxiv : the preprint server for biology · 2023Article
- A polycistronic transgene design for combinatorial genetic perturbations from a single transcript in Drosophila.PLoS genetics · 2023Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In Drosophila, the Gal4-UAS system permits a transgene to be expressed in the same pattern as a gene of interest by placing the Gal4 transcription factor under control of the gene's DNA regulatory elements. If these regulatory elements are not known, however, expression of Gal4 in the desired pattern may be difficult or impossible. To solve this problem, we have developed a method for co-expressing Gal4 with the endogenous gene by exploiting the "ribosomal skipping" mechanism of the viral T2A peptide. This method requires explicit knowledge only of the endogenous gene's open reading frame and not its regulatory elements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.