ReviewBiochimica et biophysica acta2012
High resolution electrophysiological techniques for the study of calcium-activated exocytosis.
Review in Biochimica et biophysica acta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Lytic granule exocytosis at immune synapses: lessons from neuronal synapses.Frontiers in immunology · 2023Review
- A Nanodisc-Cell Fusion Assay with Single-Pore Sensitivity and Sub-millisecond Time Resolution.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Individual synaptic vesicles mediate stimulated exocytosis from cochlear inner hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- Expression, purification, and electrophysiological characterization of a recombinant, fluorescent Kir6.2 in mammalian cells.Protein expression and purification · 2018Article
- The Dual Function of the Polybasic Juxtamembrane Region of Syntaxin 1A in Clamping Spontaneous Release and Stimulating CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men.Physiological reviews · 2018Review
- Article
- Regulation of Exocytotic Fusion Pores by SNARE Protein Transmembrane Domains.Frontiers in molecular neuroscience · 2017Review
- Reconstruction of membrane current by deconvolution and its application to membrane capacitance measurements in cardiac myocytes.PloS one · 2017Article
- Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains.Scientific reports · 2016Article
- Using ApoE Nanolipoprotein Particles To Analyze SNARE-Induced Fusion Pores.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Non-Faradaic Electrochemical Detection of Exocytosis from Mast and Chromaffin Cells Using Floating-Gate MOS Transistors.Scientific reports · 2015Article
- A microfluidic platform for chemical stimulation and real time analysis of catecholamine secretion from neuroendocrine cells.Lab on a chip · 2013Article
- High-resolution membrane capacitance measurements for the study of exocytosis and endocytosis.Nature protocols · 2013Article
- Signaling from the secretory granule to the nucleus.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
1 author at 1 institution in 2 countries.
Funding
Abstract
backgroundNeurotransmitters, neuropeptides and hormones are released from secretory vesicles of nerve terminals and neuroendocrine cells by calcium-activated exocytosis. A key step in this process is the formation of a fusion pore between the vesicle membrane and the plasma membrane. Exocytotic fusion leads to an increase in plasma membrane area that can be measured as a proportional increase in plasma membrane capacitance. SCOPE OF REVIEW: High resolution capacitance measurements in single cells, nerve terminals and small membrane patches have become possible with the development of the patch clamp technique. This review discusses the methods of whole cell patch clamp capacitance measurements and their use in conjunction with voltage clamp pulse stimulation and with stimulation by photorelease of caged calcium. It also discusses patch capacitance measurements for the study of single exocytotic events and fusion pore properties in neuroendocrine cells and nerve terminals. MAJOR
conclusionsCapacitance measurements provide high resolution information on the extent and time course of fusion for the characterization of vesicle pools and the kinetics of exocytosis. They allow the characterization of the mode of fusion including distinction of single vesicle full fusion, transient kiss-and-run fusion or multivesicular compound exocytosis. Furthermore, measurement of fusion pore conductances and their dynamic behavior has enabled the characterization of fusion pore properties in a way that resembles the characterization of ion channel function through single channel recordings. GENERAL SIGNIFICANCE: The combination of patch clamp capacitance measurements with pharmacological and molecular manipulations of exocytosis is emerging as a powerful approach to investigate the molecular mechanisms of calcium-activated exocytotic fusion pore formation. This article is part of a Special Issue entitled Biochemical, biophysical and genetic approaches to intracellular calcium signalling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.