ArticleCurrent biology : CB2012
Mad2 and Mad3 cooperate to arrest budding yeast in mitosis.
Article in Current biology : CB, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- The vacuole shapes the nucleus and the ribosomal DNA loop during mitotic delays.Life science alliance · 2022Article
- Distinct requirements for the COMPASS core subunits Set1, Swd1, and Swd3 during meiosis in the budding yeast Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2021Article
- Cell-cycle phospho-regulation of the kinetochore.Current genetics · 2021Review
- Deficiency of PTEN leads to aberrant chromosome segregation through downregulation of MAD2.Molecular medicine reports · 2019Article
- Electron cryotomography analysis of Dam1C/DASH at the kinetochore-spindle interface in situ.The Journal of cell biology · 2019Article
- Cell-Cycle Regulation of Dynamic Chromosome Association of the Condensin Complex.Cell reports · 2018Article
- C-terminal region of Mad2 plays an important role during mitotic spindle checkpoint in fission yeast Schizosaccharomyces pombe.Molecular biology reports · 2017Article
- Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions.G3 (Bethesda, Md.) · 2016Article
- Article
- Signalling dynamics in the spindle checkpoint response.Nature reviews. Molecular cell biology · 2014Review
- Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- WT1 interacts with MAD2 and regulates mitotic checkpoint function.Nature communications · 2014Article
- RNAi silencing of the SoxE gene suppresses cell proliferation in silkworm BmN4 cells.Molecular biology reports · 2014Article
- Chromosome segregation in budding yeast: sister chromatid cohesion and related mechanisms.Genetics · 2014Review
- The dynamics of signal amplification by macromolecular assemblies for the control of chromosome segregation.Frontiers in physiology · 2014Review
- The C-terminal domains of human neurofibromin and its budding yeast homologs Ira1 and Ira2 regulate the metaphase to anaphase transition.Cell cycle (Georgetown, Tex.) · 2014Article
- Monopolar spindle 1 (MPS1) kinase promotes production of closed MAD2 (C-MAD2) conformer and assembly of the mitotic checkpoint complex.The Journal of biological chemistry · 2013Article
- Review
- Catalytic assembly of the mitotic checkpoint inhibitor BubR1-Cdc20 by a Mad2-induced functional switch in Cdc20.Molecular cell · 2013Article
- Panta rhei: the APC/C at steady state.The Journal of cell biology · 2013Review
Corrections and comments
- Commented on by
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe spindle checkpoint ensures accurate chromosome transmission by delaying chromosome segregation until all chromosomes are correctly aligned on the mitotic spindle. The checkpoint is activated by kinetochores that are not attached to microtubules or are attached but not under tension and arrests cells at metaphase by inhibiting the anaphase-promoting complex (APC) and its coactivator Cdc20. Despite numerous studies, we still do not understand how the checkpoint proteins coordinate with each other to inhibit APC(Cdc20) activity.
resultsTo ask how the checkpoint components induce metaphase arrest, we constructed fusions of checkpoint proteins and expressed them in the budding yeast Saccharomyces cerevisiae to mimic possible protein interactions during checkpoint activation. We found that expression of a Mad2-Mad3 protein fusion or noncovalently linked Mad2 and Mad3, but not the overexpression of the two separate proteins, induces metaphase arrest that is independent of functional kinetochores or other checkpoint proteins. We further showed that artificially tethering Mad2 to Cdc20 also arrests cells in metaphase independently of other checkpoint components.
conclusionOur results suggest that Mad3 is required for the stable binding of Mad2 to Cdc20 in vivo, which is sufficient to inhibit APC activity and is the most downstream event in spindle checkpoint activation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.