ArticleBiomicrofluidics2011
Microfluidic concentration of bacteria by on-chip electrophoresis.
Article in Biomicrofluidics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- Progress of Microfluidic Continuous Separation Techniques for Micro-/Nanoscale Bioparticles.Biosensors · 2021Review
- A new approach to identifying pathogens, with particular regard to viruses, based on capillary electrophoresis and other analytical techniques.Trends in analytical chemistry : TRAC · 2021Review
- Analysis of pressure-driven membrane preconcentration for point-of-care assays.Biomicrofluidics · 2020Article
- Microfluidics-Based Organism Isolation from Whole Blood: An Emerging Tool for Bloodstream Infection Diagnosis.Annals of biomedical engineering · 2019Review
- Early detection of bacteria using SPR imaging and event counting: experiments withRSC advances · 2019Article
- Diagnosis of Bacterial Pathogens in the Urine of Urinary-Tract-Infection Patients Using Surface-Enhanced Raman Spectroscopy.Molecules (Basel, Switzerland) · 2018Article
- A nanofilter for fluidic devices by pillar-assisted self-assembly microparticles.Biomicrofluidics · 2018Article
- Article
- Microfluidic cell concentrator with a reduced-deviation-flow herringbone structure.Biomicrofluidics · 2017Article
- A Protein Nanopore-Based Approach for Bacteria Sensing.Nanoscale research letters · 2016Article
- Deformability-Based Electrokinetic Particle Separation.Micromachines · 2016Article
- Fabrication of anti-protein-fouling poly(ethylene glycol) microfluidic chip electrophoresis by sandwich photolithography.Biomicrofluidics · 2016Article
- Fabrication of universal serial bus flash disk type microfluidic chip electrophoresis and application for protein analysis under ultra low voltage.Biomicrofluidics · 2016Article
- Hydrogel-based microfluidic incubator for microorganism cultivation and analyses.Biomicrofluidics · 2015Article
- Continuous enrichment of low-abundance cell samples using standing surface acoustic waves (SSAW).Lab on a chip · 2014Article
- Simplified confocal microscope for counting particles at low concentrations.The Review of scientific instruments · 2013Article
- Optoacoustic tweezers: a programmable, localized cell concentrator based on opto-thermally generated, acoustically activated, surface bubbles.Lab on a chip · 2013Article
- Microfluidic rectifier based on poly(dimethylsiloxane) membrane and its application to a micropump.Biomicrofluidics · 2013Article
- On-chip microfluidic biosensor using superparamagnetic microparticles.Biomicrofluidics · 2013Article
- Convenient quantification of methanol concentration detection utilizing an integrated microfluidic chip.Biomicrofluidics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this contribution, we present a system for efficient preconcentration of pathogens without affecting their viability. Development of miniaturized molecular diagnostic kits requires concentration of the sample, molecule extraction, amplification, and detection. In consequence of low analyte concentrations in real-world samples, preconcentration is a critical step within this workflow. Bacteria and viruses exhibit a negative surface charge and thus can be electrophoretically captured from a continuous flow. The concept of phaseguides was applied to define gel membranes, which enable effective and reversible collection of the target species. E. coli of the strains XL1-blue and K12 were used to evaluate the performance of the device. By suppression of the electroosmotic flow both strains were captured with efficiencies of up to 99%. At a continuous flow of 15 μl/min concentration factors of 50.17 ± 2.23 and 47.36 ± 1.72 were achieved in less than 27 min for XL1-blue and K12, respectively. These results indicate that free flow electrophoresis enables efficient concentration of bacteria and the presented device can contribute to rapid analyses of swab-derived samples.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.