ArticleHistochemistry and cell biology2012
Epithelial sodium channels (ENaC) are uniformly distributed on motile cilia in the oviduct and the respiratory airways.
Article in Histochemistry and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 108 citations in OpenAlex.
- Genetic Risk Factors for Nontuberculous Mycobacterial Pulmonary Disease (Systematic Review).Sovremennye tekhnologii v meditsine · 2024Pooled it
- Drift and Selection in a Recently Colonized Sea: The Genomic Basis of Adaptation to Low-Salinity Waters in Baltic Harbour Porpoises.Molecular ecology · 2026Article
- Amiloride mitigates respiratory distress caused by WFDC2 deficiency via inhibiting the epithelial sodium channel.Nature communications · 2026Article
- Case Report: Compound Heterozygous SCNN1B Mutations Causing Pseudohypoaldosteronism Type 1B2 in Neonatal Twins.Molecular genetics & genomic medicine · 2026Article
- Characterisation of a primary ciliary dyskinesia model generated from BMI1-transduced basal epithelial cells.Journal of cell science · 2025Article
- Ion-Channel Proteins in the Prepubertal Bitch Reproductive System: The Immunolocalization of ASIC2, ASIC4, and PIEZO2.International journal of molecular sciences · 2025Article
- N-carbamylglutamate supplementation improves laying performance of layers by regulating hypothalamic-pituitary-ovarian axis.Frontiers in veterinary science · 2025Article
- Review
- Review
- Epithelial NaComprehensive Physiology · 2024Review
- Human pancreatic ductal organoids with controlled polarity provide a novel ex vivo tool to study epithelial cell physiology.Cellular and molecular life sciences : CMLS · 2023Article
- The impact of primary ciliary dyskinesia on female and male fertility: a narrative review.Human reproduction update · 2023Review
- The Epithelial Sodium Channel-An Underestimated Drug Target.International journal of molecular sciences · 2023Review
- Association of Neonatal Hospital Length of Stay with Lung Function in Primary Ciliary Dyskinesia.Annals of the American Thoracic Society · 2022Article
- Bioelectric Signaling: Role of Bioelectricity in Directional Cell Migration in Wound Healing.Cold Spring Harbor perspectives in biology · 2022Review
- Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems.Cell reports methods · 2022Article
- ENaC activation by proteases.Acta physiologica (Oxford, England) · 2022Review
- Essentials in saline pharmacology for nasal or respiratory hygiene in times of COVID-19.European journal of clinical pharmacology · 2021Review
- Review
- Metachronal μ-Cilia for On-Chip Integrated Pumps and Climbing Robots.ACS applied materials & interfaces · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epithelial sodium channels (ENaCs) are located on the apical surface of cells and funnel Na(+) ions from the lumen into the cell. ENaC function also regulates extracellular fluid volume as water flows across membranes accompanying Na(+) ions to maintain osmolarity. To examine the sites of expression and intracellular localization of ENaC, we generated polyclonal antibodies against the extracellular domain of human α-ENaC subunit that we expressed in E. coli. Three-dimensional (3D) confocal microscopy of immunofluorescence using these antibodies for the first time revealed that ENaCs are uniformly distributed on the ciliary surface in all epithelial cells with motile cilia lining the bronchus in human lung and female reproductive tract, all along the fimbrial end of the fallopian tube, the ampulla and rare cells in the uterine glands. Quantitative analysis indicated that cilia increase cell surface area >70-fold and the amount of ENaC on cilia is >1,000-fold higher than on non-ciliated cell surface. These findings indicate that ENaC functions as a regulator of the osmolarity of the periciliary fluid bathing the cilia. In contrast to ENaC, cystic fibrosis transmembrane conductance regulator (CFTR) that channels chloride ions from the cytoplasm to the lumen is located mainly on the apical side, but not on cilia. The cilial localization of ENaC requires reevaluation of the mechanisms of action of CFTR and other modulators of ENaC function. ENaC on motile cilia should be essential for diverse functions of motile cilia, such as germ cell transport, fertilization, implantation, clearance of respiratory airways and cell migration.
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