ArticleDevelopmental biology2012
Oocyte specific oolemmal SAS1B involved in sperm binding through intra-acrosomal SLLP1 during fertilization.
Article in Developmental biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 57 citations in OpenAlex.
- Crossing the barrier or how regulation of ovastacin controls fertilization and translates into clinical phenotypes.iScience · 2025Review
- Protein profile of spermatozoa and seminal plasma based on molecular weight in four phenotypes ofOpen veterinary journal · 2025Article
- Cancer-oocyte SAS1B protein is expressed at the cell surface of multiple solid tumors and targeted with antibody-drug conjugates.Journal for immunotherapy of cancer · 2024Article
- Ovastacin: An oolemma protein that cleaves the zona pellucida to prevent polyspermy.Clinical and experimental reproductive medicine · 2023Article
- Combined proteomics and transcriptomics identifies serpin family C member 1 associated protein as a biomarker of endometriosis.Annals of medicine · 2023Article
- Influence of Risk Factors for Male Infertility on Sperm Protein Composition.International journal of molecular sciences · 2021Review
- Molecular mechanisms and evolution of fertilization proteins.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2021Review
- Role of Integrins in Sperm Activation and Fertilization.International journal of molecular sciences · 2021Review
- Electroporation of Mouse Follicles, Oocytes and Embryos without Manipulating Zona Pellucida.Journal of developmental biology · 2021Article
- Mammalian egg coat modifications and the block to polyspermy.Molecular reproduction and development · 2020Review
- Flutamide-induced alterations in transcriptional profiling of neonatal porcine ovaries.Journal of animal science and biotechnology · 2019Article
- Evaluation of SAS1B as a target for antibody-drug conjugate therapy in the treatment of pancreatic cancer.Oncotarget · 2018Article
- Intracellular activation of ovastacin mediates pre-fertilization hardening of the zona pellucida.Molecular human reproduction · 2017Article
- Zinc sparks induce physiochemical changes in the egg zona pellucida that prevent polyspermy.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- LYZL6, an acidic, bacteriolytic, human sperm-related protein, plays a role in fertilization.PloS one · 2017Article
- Effect of insulin supplementation on in vitro maturation of pre-antral follicles from adult and pre-pubertal mice.In vitro cellular & developmental biology. Animal · 2016Article
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- Membrane associated cancer-oocyte neoantigen SAS1B/ovastacin is a candidate immunotherapeutic target for uterine tumors.Oncotarget · 2015Article
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Corrections and comments
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
Abstract
Molecular mechanisms by which fertilization competent acrosome-reacted sperm bind to the oolemma remain uncharacterized. To identify oolemmal binding partner(s) for sperm acrosomal ligands, affinity panning was performed with mouse oocyte lysates using sperm acrosomal protein, SLLP1 as a target. An oocyte specific membrane metalloproteinase, SAS1B (Sperm Acrosomal SLLP1 Binding), was identified as a SLLP1 binding partner. cDNA cloning revealed six SAS1B splice variants, each containing a zinc binding active site and a putative transmembrane domain, with signal peptides in three variants. SAS1B transcripts were ovary specific. SAS1B protein was first detected in early secondary follicles in day 3 ovaries. Immunofluorescence localized SAS1B to the microvillar oolemma of M2 oocytes. After fertilization, SAS1B decreased on the oolemma and became virtually undetectable in blastocysts. In transfected CHO-K1 cells SAS1B localized to the surface of unpermeabilized cells. Recombinant and native SLLP1 co-localized with SAS1B to the microvillar domain of ovulated M2 oocytes. Molecular interactions between mouse SLLP1 and SAS1B were demonstrated by surface plasmon resonance, far-western, yeast two-hybrid, recombinant- and native-co-IP analyses. SAS1B bound to SLLP1 with high affinity. SAS1B had protease activity, and SAS1B protein or antibody significantly inhibited fertilization. SAS1B knockout female mice showed a 34% reduction in fertility. The study identified SAS1B-SLLP1 as a pair of novel sperm-egg binding partners involving the oolemma and intra-acrosomal compartment during fertilization.
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Registered trials
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