Evidence map›Paper›PMID 22206011›Full record

ArticlePloS one2011

Identification of Eps15 as antigen recognized by the monoclonal antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila brain.

Partho Halder, Yi-Chun Chen, Janine Brauckhoff, Alois Hofbauer, Marie-Christine Dabauvalle, Urs Lewandrowski, Christiane Winkler, Albert Sickmann, Erich Buchner

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Synapsin function in GABA-ergic interneurons is required for short-term olfactory habituation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Partho HalderDepartment of Neurobiology and Genetics, Theodor-Boveri Institute, University of Wuerzburg, Wuerzburg, Germany.
Yi-Chun Chen
Janine Brauckhoff
Alois Hofbauer
Marie-Christine Dabauvalle
Urs Lewandrowski
Christiane Winkler
Albert Sickmann
Erich Buchner
University of Würzburg · DEUniversity of Regensburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.

Indexed as

HybridomasAnimalsAntibodies, MonoclonalAntigensBrainDrosophila melanogasterDrosophila ProteinsElectrophoresis, Polyacrylamide GelImmunoprecipitationIntracellular SpaceMass SpectrometryNerve Tissue ProteinsProtein Structure, TertiaryProtein TransportStaining and LabelingAntibodies, MonoclonalAntigensDrosophila ProteinsEps-15 protein, DrosophilaNerve Tissue Proteins

Identifiers

PMID22206011
PMCPMC3244249
OpenAlexW2071638989

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.