ArticleJournal of clinical microbiology2012
Pooling nasopharyngeal/throat swab specimens to increase testing capacity for influenza viruses by PCR.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed.
- Bayesian Additive Regression Trees for Group Testing Data.Statistics in medicine · 2025Article
- Transmission of Swine Influenza A Viruses along Pig Value Chains, Cambodia, 2020-2022.Emerging infectious diseases · 2024Article
- Evaluation of Fecal Sample Pooling for Real-Time RT-PCR Testing SARS-CoV-2 in Animals.Viruses · 2024Article
- Additive partially linear model for pooled biomonitoring data.Computational statistics & data analysis · 2024Article
- binGroup2: Statistical Tools for Infection Identification via Group Testing.The R journal · 2023Article
- Accelerating the Laboratory Testing Capacity through Saliva Pooling Prior to Direct RT-qPCR for SARS-CoV-2 Detection.Diagnostics (Basel, Switzerland) · 2022Article
- Capturing the pool dilution effect in group testing regression: A Bayesian approach.Statistics in medicine · 2022Article
- Farm management practices, biosecurity and influenza a virus detection in swine farms: a comprehensive study in colombia.Porcine health management · 2022Article
- Approaches to Deployment of Molecular Testing for SARS-CoV-2 in Resource-Limited Settings.Clinics in laboratory medicine · 2022Review
- Article
- Pooled sample testing for COVID-19 diagnosis: Evaluation of bi-directional matrix pooling strategies.Journal of virological methods · 2022Article
- Boosting SARS-CoV-2 detection combining pooling and multiplex strategies.Scientific reports · 2022Article
- Evaluation of SARS-CoV-2 genome detection by real-time PCR assay using pooled specimens.Future virology · 2022Article
- A four specimen-pooling scheme reliably detects SARS-CoV-2 and influenza viruses using the BioFire FilmArray Respiratory Panel 2.1.Scientific reports · 2022Article
- Detection of SARS-CoV-2 RNA in Upper Respiratory Swap Samples by Pooling MethodBalkan medical journal · 2022Article
- SARS-CoV-2 detection in multi-sample pools in a real pandemic scenario: A screening strategy of choice for active surveillance.PloS one · 2022Article
- Pooling saliva samples as an excellent option to increase the surveillance for SARS-CoV-2 when re-opening community settings.PloS one · 2022Article
- Article
- Safe and effective pool testing for SARS-CoV-2 detection.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2021Article
- Generalized additive regression for group testing data.Biostatistics (Oxford, England) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Real-time PCR methodology can be applied to rapidly and accurately detect influenza viruses. During times of surge testing or enhanced pandemic surveillance, public health laboratories (PHLs) may experience overwhelming demand for testing, even while the prevalence of positive specimens remains low. To improve laboratory capacity and testing efficiency during surges, we evaluated whether nasopharyngeal (NP)/throat swab specimens can be pooled and tested for the presence of the 2009 H1N1 influenza virus without a reduction in sensitivity. Pools of 10 specimens were extracted and concentrated upon elution on the MagNA Pure LC instrument, and real-time PCR was performed on the Applied Biosystems 7500 Fast platform, using the CDC swine influenza virus real-time RT-PCR detection panel (rRT-PCR swine flu panel). Specimens in positive pools were singly re-extracted and retested by PCR to identify individual positive samples. Initial studies showed that spiking a pool of nine negative specimens (100 μl each) or 900 μl of virus transport medium with 100 μl of a positive clinical specimen caused no loss of sensitivity by rRT-PCR testing. Pools containing either multiple positive specimens or specimens positive for other respiratory viruses also showed no negative effect on crossing threshold (C(T)) values. To test the robustness of the pooling protocol, a panel of 50 blinded samples was sent to three PHLs and tested in five pools of 10. All PHLs correctly identified the positive specimens. This study demonstrates the feasibility of using a pooling strategy to increase capacity and conserve resources during surge testing and periods of enhanced influenza surveillance when the prevalence is low.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.