ArticleJournal of clinical microbiology2012
Legionella pneumophila sequence type 1/Paris pulsotype subtyping by spoligotyping.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Comparison of whole genome sequencing typing tools for the typing of Belgian Legionella pneumophila outbreaks isolates.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- CRISPR-Cas systems: role in cellular processes beyond adaptive immunity.Folia microbiologica · 2022Review
- Legionella pneumophila Cas2 Promotes the Expression of Small Heat Shock Protein C2 That Is Required for Thermal Tolerance and Optimal Intracellular Infection.Infection and immunity · 2022Article
- Presence of Viable, Clinically RelevantMicrobiology spectrum · 2022Article
- Legionella pneumophila CRISPR-Cas Suggests Recurrent Encounters with One or More Phages in the FamilyApplied and environmental microbiology · 2021Article
- Comparison of Whole-Genome Sequences of Legionella pneumophila in Tap Water and in Clinical Strains, Flint, Michigan, USA, 2016.Emerging infectious diseases · 2019Article
- Legionella SBT applied directly to respiratory samples as a rapid molecular epidemiological tool.Scientific reports · 2019Article
- Population structure of Environmental and Clinical Legionella pneumophila isolates in Catalonia.Scientific reports · 2018Article
- Pathogenomics of Virulence Traits ofFrontiers in microbiology · 2018Article
- Genomic heterogeneity differentiates clinical and environmental subgroups of Legionella pneumophila sequence type 1.PloS one · 2018Article
- Clusters of Healthcare-Associated Legionnaires' Disease in Two Hospitals of Central Greece.Case reports in infectious diseases · 2018Article
- Epidemiological information is key when interpreting whole genome sequence data - lessons learned from a largeEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2017Article
- Differential Proteome Between Patient-Related and Non-related Environmental Isolates of Legionella pneumophila.Current microbiology · 2017Article
- High prevalence of Legionella in non-passenger merchant vessels.Epidemiology and infection · 2017Article
- Benefits of Genomic Insights and CRISPR-Cas Signatures to Monitor Potential Pathogens across Drinking Water Production and Distribution Systems.Frontiers in microbiology · 2017Article
- Multiplication of Legionella pneumophila Sequence Types 1, 47, and 62 in Buffered Yeast Extract Broth and Biofilms Exposed to Flowing Tap Water at Temperatures of 38°C to 42°C.Applied and environmental microbiology · 2016Article
- SpoTyping: fast and accurate in silico Mycobacterium spoligotyping from sequence reads.Genome medicine · 2016Article
- Current and emerging Legionella diagnostics for laboratory and outbreak investigations.Clinical microbiology reviews · 2015Review
- Real-time investigation of a Legionella pneumophila outbreak using whole genome sequencing.Epidemiology and infection · 2014Article
- Validation of a microbead-based format for spoligotyping of Legionella pneumophila.Journal of clinical microbiology · 2014Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endemic strains of Legionella pneumophila sequence type 1 (ST1), in particular the ST1/Paris pulsotype, are dispersed worldwide and represent about 10% of culture-proven clinical cases of Legionnaires' disease in France. The high rate of isolation of this strain from both clinical and environmental samples makes identification of the source of infection difficult during epidemiological investigations. The full-length genome sequence of this strain was recently determined, and it revealed the presence of a CRISPR/cas complex. The aim of this study was to develop and evaluate a spoligotyping tool based on the diversity of this CRISPR locus that would allow the accurate subtyping of the L. pneumophila serogroup 1 ST1/Paris pulsotype. The CRISPR loci of 28 L. pneumophila ST1/Paris pulsotype isolates were sequenced, and 42 different spacers regions were characterized. A membrane-based spoligotyping method was developed and used to determine the subtypes of 406 L. pneumophila isolates, including 233 with the ST1/Paris pulsotype profile that were collected in France from 2000 to 2011. A total of 46 different spoligotypes were detected, and 41 of these were specifically identified in the ST1/Paris pulsotype isolates. In 27 of 33 epidemiological investigations, the environmental source of contamination was confirmed by comparing spoligotypes of clinical isolates with those of environmental isolates. With an index of discrimination of 79.72% (95% confidence interval, 75.82 to 83.63), spoligotyping of the L. pneumophila ST1/Paris pulsotype has the potential to be a useful complementary genotyping tool for discriminating isolates with undistinguishable pulsed-field gel electrophoresis (PFGE) and ST genotypes, which could help to identify environmental sources of infection.
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