ArticleJournal of clinical microbiology2012
Genotype 3 diversity and quantification of hepatitis E virus RNA.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Hepatitis E Virus Infection in Pregnant Women.Microorganisms · 2026Review
- Host-interferon-stimulated gene response to virus-host recombinant variants of hepatitis E virus and enhanced viral replication.Microbiology spectrum · 2026Article
- Longitudinal Follow-Up of microRNA Expression During Hepatitis E Virus Infection in Immunocompromised Hosts.Biomolecules · 2026Article
- Evaluation of a New Automated Platform for the Detection and Quantification of HEV RNA.Journal of medical virology · 2026Article
- Exacerbation of Anti-Cytomegalovirus Immunity and Mobilization of γ9δ1 T Cells During the Acute Phase of Hepatitis E Virus Infection.Microbiology and immunology · 2026Article
- Characterization of virus‒host recombinant variants of the hepatitis E virus.Journal of virology · 2024Article
- Relevance of Tacrolimus Trough Concentration and Hepatitis E virus Genetic Changes in Kidney Transplant Recipients With Chronic Hepatitis E.Kidney international reports · 2024Article
- Article
- Optimization of the molecular diagnosis of the acute hepatitis E virus infection.Microbial biotechnology · 2023Article
- Article
- Seven years (2015-2021) of blood donor screening for HEV-RNA in France: lessons and perspectives.Blood transfusion = Trasfusione del sangue · 2023Article
- Article
- Broadly Reactive Real-Time RT-PCR Assay for the Detection of Hepatitis E Virus and Simultaneous Genotyping by Single Nucleotide Polymorphism Analysis.Microbiology spectrum · 2022Article
- Study of Hepatitis E Virus-4 Infection in Human Liver-Chimeric, Immunodeficient, and Immunocompetent Mice.Frontiers in microbiology · 2022Article
- Effector memory CD8 T cell response elicits Hepatitis E Virus genotype 3 pathogenesis in the elderly.PLoS pathogens · 2021Article
- Development and Clinical Validation of a Pangenotypic PCR-Based Assay for the Detection and Quantification of Hepatitis E Virus (Journal of clinical microbiology · 2021Article
- Dynamics of Hepatitis E Virus (HEV) Antibodies and Development of a Multifactorial Model To Improve the Diagnosis of HEV Infection in Resource-Limited Settings.Journal of clinical microbiology · 2021Article
- Risk for Hepatitis E Virus Transmission by Solvent/Detergent-Treated Plasma.Emerging infectious diseases · 2020Article
- Optimized Hepatitis E Virus (HEV) Culture and its Application to Measurements of HEV Infectivity.Viruses · 2020Article
- Insertions and Duplications in the Polyproline Region of the Hepatitis E Virus.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genotype 3 hepatitis E viruses (HEVs) are distributed across the world and are now considered to be an emerging public health concern in industrialized countries. At least 10 genotype 3 subtypes have been identified in humans and animals worldwide. It was recently reported that the sensitivities of HEV RNA assays differ greatly. We have assessed the influence of genotype 3 diversity on the performances of two HEV RNA assays: one targeting the ORF3 gene and the other targeting the ORF2 gene. We tested a panel of 5 HEV-positive reference samples of genotypes 3a, 3b, 3c, 3e, and 3f at 10-fold serial dilutions. The HEV RNA concentrations obtained with both reverse transcription (RT)-PCRs were correlated, but the RT-PCR based on ORF2 underestimated the HEV RNA concentrations. The mean [ORF3 - ORF2] difference was 1.41 log copies/ml. We also tested 34 clinical specimens of genotypes 3c (n = 15), 3e (n = 4), and 3f (n = 15), representing the most prevalent subtypes in Europe. The mean [ORF3 - ORF2] differences were 1.41 log copies/ml for genotype 3c, 0.96 log copies/ml for genotype 3e, and 0.70 log copies/ml for genotype 3f. The bias between the 2 RT-PCR assays was significantly greater for genotype 3c than for genotype 3f (P = 0.007). We therefore recommend the use of an RT-PCR protocol based on ORF3 to quantify HEV RNA of genotype 3 strains.
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