ArticleInvestigative ophthalmology & visual science2012
Cyclosporine immunomodulation retards regeneration of surgically transected corneal nerves.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Vitamin abnormalities in neuropathic corneal pain.Eye and vision (London, England) · 2025Review
- Article
- Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals.International journal of molecular sciences · 2023Article
- A synthetic tear protein resolves dry eye through promoting corneal nerve regeneration.Cell reports · 2022Article
- Transgenic models for investigating the nervous system: Currently available neurofluorescent reporters and potential neuronal markers.Biochimica et biophysica acta. General subjects · 2020Review
- Allosteric Cannabinoid Receptor 1 (CB1) Ligands Reduce Ocular Pain and Inflammation.Molecules (Basel, Switzerland) · 2020Article
- Corneal nerve healing after in situ laser nerve transection.PloS one · 2019Article
- Corneal nerve regeneration after herpes simplex keratitis: A longitudinal in vivo confocal microscopy study.The ocular surface · 2018Article
- Treatment outcomes in the DRy Eye Amniotic Membrane (DREAM) study.Clinical ophthalmology (Auckland, N.Z.) · 2018Article
- Clinical Efficacy of Platelet-Rich Plasma in the Treatment of Neurotrophic Corneal Ulcer.Journal of ophthalmology · 2018Article
- Neuropathic Corneal Pain: Approaches for Management.Ophthalmology · 2017Review
- PEDF plus DHA modulate inflammation and stimulate nerve regeneration after HSV-1 infection.Experimental eye research · 2017Article
- Corneal Nerve Regeneration after Self-Retained Cryopreserved Amniotic Membrane in Dry Eye Disease.Journal of ophthalmology · 2017Article
- Understanding Neuropathic Corneal Pain--Gaps and Current Therapeutic Approaches.Seminars in ophthalmology · 2016Review
- The PEDF Neuroprotective Domain Plus DHA Induces Corneal Nerve Regeneration After Experimental Surgery.Investigative ophthalmology & visual science · 2015Article
- CD11b+GR1+ myeloid cells secrete NGF and promote trigeminal ganglion neurite growth: implications for corneal nerve regeneration.Investigative ophthalmology & visual science · 2013Article
- Ocular surface extracellular DNA and nuclease activity imbalance: a new paradigm for inflammation in dry eye disease.Investigative ophthalmology & visual science · 2012Article
- Semaphorin 7a links nerve regeneration and inflammation in the cornea.Investigative ophthalmology & visual science · 2012Article
- Corneal neurotoxicity due to topical benzalkonium chloride.Investigative ophthalmology & visual science · 2012Article
- What Causes Eye Pain?Current ophthalmology reportsArticle
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo determine whether immunomodulation with cyclosporine (CsA) affects reinnervation after surgical transection of stromal nerves.
methodsThy1-YFP+ neurofluorescent mice underwent lamellar corneal surgery and 3 days later, received artificial tears or CsA eye drops for 6 weeks. Serial in vivo wide-field stereofluorescent microscopy was performed to determine changes in nerve fiber density (NFD). Real-time quantitative PCR was performed to determine the expression of neurotrophins and cytokines (IL6 and TNF-α). Compartmental culture of trigeminal ganglion neurons was performed in Campenot devices to determine whether CsA directly affects neurite outgrowth.
resultsYellow fluorescent protein (YFP)-positive cells significantly increased at 3 and 7 days after surgery. The number of YFP-positive cells in the cornea was significantly lower in the CsA group than that in the control group. The percentage increase in NFD between 2 to 6 weeks was greater in the control group (80% ± 10%, P = 0.05) than that in the CsA group (39% ± 21%). The CsA group also exhibited lower expression of IL6 and TNF-α (P = 0.01). In compartmental culture experiments, neurite outgrowth toward side compartments containing CsA was significantly less (2.29 ± 0.4 mm, P = 0.01) than that toward side compartments containing vehicle (3.97 ± 0.71 mm).
conclusionsImmunomodulation with CsA reduces the expression of cytokines (IL6) in the cornea and retards regenerative sprouting from transected corneal stromal nerve trunks. In addition, CsA has a direct growth inhibitory action on neurites as well.
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Registered trials
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