ArticlePlanta2012
Induction of potato steroidal glycoalkaloid biosynthetic pathway by overexpression of cDNA encoding primary metabolism HMG-CoA reductase and squalene synthase.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Integrative metabolomic and transcriptomic analysis provides insight into the mechanism of pigmentation in potato tuber eyes.BMC plant biology · 2025Article
- Genome-wide association study identifies candidate genes for glycoalkaloid biosynthesis in tetraploid potato (Solanum tuberosum L.) tubers.BMC plant biology · 2025Article
- Potato steroidal glycoalkaloids: properties, biosynthesis, regulation and genetic manipulation.Molecular horticulture · 2024Review
- Allelic variation in the autotetraploid potato: genes involved in starch and steroidal glycoalkaloid metabolism as a case study.BMC genomics · 2024Article
- Metabolomic and transcriptomic analyses identify external conditions and key genes underlying high levels of toxic glycoalkaloids in tubers of stress-sensitive potato cultivars.Frontiers in plant science · 2023Article
- GWAS in tetraploid potato: identification and validation of SNP markers associated with glycoalkaloid content.Molecular breeding : new strategies in plant improvement · 2022Article
- Sustainable Use of Bioactive Compounds fromMolecules (Basel, Switzerland) · 2021Review
- Genome-Wide HMG Family Investigation and Its Role in Glycoalkaloid Accumulation in Wild Tuber-BearingLife (Basel, Switzerland) · 2020Article
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- Molecular Cloning and Functional Identification of a Squalene Synthase Encoding Gene from Alfalfa (International journal of molecular sciences · 2019Article
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- Cloning, Expression Analysis and Functional Characterization of Squalene Synthase (SQS) from Tripterygium wilfordii.Molecules (Basel, Switzerland) · 2018Article
- Lanosterol synthase-like is involved with differential accumulation of steroidal glycoalkaloids in potato.Planta · 2017Article
- Genome diversity of tuber-bearingProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Identification of genes related to skin development in potato.Plant molecular biology · 2017Article
- Transcriptome analysis reveals metabolic alteration due to consecutive monoculture and abiotic stress stimuli in Rehamannia glutinosa Libosch.Plant cell reports · 2017Article
- The Solanum tuberosum KST1 partial promoter as a tool for guard cell expression in multiple plant species.Journal of experimental botany · 2017Article
- Transcript profiling of two potato cultivars during glycoalkaloid-inducing treatments shows differential expression of genes in sterol and glycoalkaloid metabolism.Scientific reports · 2017Article
- Integrated RNA-seq and sRNA-seq analysis reveals miRNA effects on secondary metabolism in Solanum tuberosum L.Molecular genetics and genomics : MGG · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Potato steroidal glycoalkaloids (SGAs) are toxic secondary metabolites whose total content in tubers must be regulated. SGAs are biosynthesized by the sterol branch of the mevalonic acid/isoprenoid pathway. In a previous study, we showed a correlation between SGA levels and the abundance of transcript coding for HMG-CoA reductase 1 (HMG1) and squalene synthase 1 (SQS1) in potato tissues and potato genotypes varying in SGA content. Here, Solanum tuberosum cv. Desirée (low SGA producer) was transformed with a gene construct containing the coding region of either HMG1 or SQS1 of Solanum chacoense Bitt. clone 8380-1, a high SGA producer. SGA levels in transgenic HMG-plants were either greater than (in eight of 14 plants) or no different from untransformed controls, whereas only four of 12 SQS-transgenics had greater SGA levels than control, as determined by HPLC. Quantitative real-time PCR was used to estimate relative steady-state transcript levels of isoprenoid-, steroid-, and SGA-related genes in leaves of the transgenic plants compared to nontransgenic controls. HMG-transgenic plants exhibited increased transcript accumulation of SQS1, sterol C24-methyltransferase type1 (SMT1), and solanidine glycosyltransferase 2 (SGT2), whereas SQS-transgenic plants, had consistently lower transcript levels of HMG1 and variable SMT1 and SGT2 transcript abundance among different transgenics. HMG-transgenic plants exhibited changes in transcript accumulation for some sterol biosynthetic genes as well. Taken together, the data suggest coordinated regulation of isoprenoid metabolism and SGA secondary metabolism.
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