ArticleCurrent genetics2012
The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza.
Article in Current genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens.Scientific reports · 2022Article
- Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.).Molecular biology reports · 2022Article
- Article
- Selection and validation of reference genes for quantitative real-time PCR in the green microalgae Tetraselmis chui.PloS one · 2021Article
- Selection of Suitable Reference Genes for qPCR Gene Expression Analysis of HepG2 and L02 in Four Different Liver Cell Injured Models.BioMed research international · 2020Article
- Selection and Validation of Reference Genes for RT-PCR Expression Analysis of Candidate Genes Involved in Morphine-Induced Conditioned Place Preference Mice.Journal of molecular neuroscience : MN · 2018Article
- Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea Amphipoda).Scientific reports · 2018Article
- Selection of reference genes for microRNA analysis associated to early stress response to handling and confinement in Salmo salar.Scientific reports · 2017Article
- Heat shock factors in carrot: genome-wide identification, classification, and expression profiles response to abiotic stress.Molecular biology reports · 2015Article
- Screening for Suitable Reference Genes for Quantitative Real-Time PCR in Heterosigma akashiwo (Raphidophyceae).PloS one · 2015Article
- Article
- Identification of a novel reference gene for apple transcriptional profiling under postharvest conditions.PloS one · 2015Article
- The green seaweed Ulva: a model system to study morphogenesis.Frontiers in plant science · 2015Article
- Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).PloS one · 2014Article
- Validation of reference genes in Solenopsis invicta in different developmental stages, castes and tissues.PloS one · 2013Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Normalization based on inappropriate reference gene may lead to the reduction of the accuracy of RT-qPCR. Although determination of suitable reference genes is essential to RT-qPCR studies, reports on the evaluation of reference genes in Ulva linza, a ubiquitous green-tide forming alga, are lacking. The expression levels of ten candidate reference genes were analyzed in U. linza across different experimental treatments, and the best-ranked reference genes differed across the treatments. The most suitable reference genes were tubulin2 (TUB2) among different salinity and UV treatments. Histone 2 (H2) was stably expressed in different temperature and desiccation stress treatments. 18S rRNA exhibited better expression stability in different light intensity treatments. While all tested samples were considered, none of single gene was widely applicable as a reference gene. Moreover, using a combination of two genes as reference genes might improve the reliability of gene expression by RT-qPCR, and the combination of TUB1 and TUB2 was selected as ideal for all tested samples. The results suggest that assessing the stability of reference gene expression patterns, determining candidates, and testing their suitability are required for each experimental investigation. The results will guide the selection of reference genes for gene expression studies in U. linza.
Indexed as
Identifiers
22205301What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.