ArticleHistochemistry and cell biology2012
STK31(TDRD8) is dynamically regulated throughout mouse spermatogenesis and interacts with MIWI protein.
Article in Histochemistry and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Tdrd15 is dispensable for male fertility and spermatogenesis in the golden hamster.European journal of medical research · 2025Article
- BRD9 functions as a methylarginine reader to regulate AKT-EZH2 signaling.Science advances · 2025Article
- The functions and mechanisms of piRNAs in mediating mammalian spermatogenesis and their applications in reproductive medicine.Cellular and molecular life sciences : CMLS · 2024Review
- Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis.BMC genomics · 2024Article
- Modern tools applied to classic structures: Approaches for mammalian male germ cell RNA granule research.Andrology · 2023Review
- Effectors and effects of arginine methylation.Biochemical Society transactions · 2023Article
- Article
- Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak.PloS one · 2020Article
- The clinical value, regulatory mechanisms, and gene network of the cancer-testis gene STK31 in pancreatic cancer.Oncotarget · 2017Article
- Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis.Asian-Australasian journal of animal sciences · 2016Article
- UPF2-Dependent Nonsense-Mediated mRNA Decay Pathway Is Essential for Spermatogenesis by Selectively Eliminating Longer 3'UTR Transcripts.PLoS genetics · 2016Article
- Identification of cell-specific targets of sumoylation during mouse spermatogenesis.Reproduction (Cambridge, England) · 2016Article
- Spata6 is required for normal assembly of the sperm connecting piece and tight head-tail conjunction.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- RAN-binding protein 9 is involved in alternative splicing and is critical for male germ cell development and male fertility.PLoS genetics · 2014Article
- Conditional inactivation of Miwi2 reveals that MIWI2 is only essential for prospermatogonial development in mice.Cell death and differentiation · 2014Article
- Article
- Mammalian piRNAs: Biogenesis, function, and mysteries.Spermatogenesis · 2014Review
- Stk31 is dispensable for embryonic development and spermatogenesis in mice.Molecular reproduction and development · 2013Article
- The Histochemistry and Cell Biology compendium: a review of 2012.Histochemistry and cell biology · 2013Review
- Characterization of MAGEG2 with testis-specific expression in mice.Asian journal of andrologyArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tudor-domain-containing proteins (TDRDs) are suggested to be critical regulators of germinal granules assembly involved in Piwi-interacting RNAs (piRNAs)-mediated pathways, of which associated components and the underlying functional mechanisms, however, remain to be elucidated. We herein characterized the expression pattern of STK31, a member of TDRDs subfamily (also termed as TDRD8), throughout spermatogenesis during mouse postnatal development. RT-PCR and Western blot verified its preferential expression in testis, but not in any other somatic tissues, in addition to embryonic stem cells. Immunofluorescent staining demonstrated that STK31 was confined to granules-like structures in mid-to-late spermatocyte cytoplasm and to acrosomal cap starting at steps 7-8 of spermatids. Furthermore, STK31 retained its localization to equatorial segment of acrosome during epididymal maturation, capacitation, and acrosome reaction. Co-immunoprecipitation assay in vivo and in vitro confirmed MIWI is a bona fide partner of STK31 in mice testes, in combination with LC/MS identification. We also discovered a group of heat shock proteins specifically associated with STK31 in vivo. Our findings suggest mouse STK31 could be a potential nuage-associated protein in the cytoplasm of mid-to-late spermatocytes and play pivotal roles related to fertilization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.