ArticleBMC research notes2011
A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Amplicon sequence proportion: A novel method for HRM primer design in DNA methylation analysis among marginalized rural population in Southern Mexico.Biology methods & protocols · 2025Article
- Second phase Chiba study of mother and child health (C-MACH): Japanese birth cohort study with multiomics analyses.BMJ open · 2024Article
- A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder.Molecular biology reports · 2024Article
- Methylomic biomarkers of lithium response in bipolar disorder: a clinical utility study.International journal of bipolar disorders · 2023Article
- The methylation profile ofFrontiers in immunology · 2023Article
- Methylomic Biomarkers of Lithium Response in Bipolar Disorder: A Proof of Transferability Study.Pharmaceuticals (Basel, Switzerland) · 2022Article
- High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia.Parasites & vectors · 2022Article
- Methylation levels assessment with Methylation-Sensitive High-Resolution Melting (MS-HRM).PloS one · 2022Article
- Global DNA methylation profile at LINE-1 repeats and promoter methylation of genes involved in DNA damage response and repair pathways in human peripheral blood mononuclear cells in response to γ-radiation.Molecular and cellular biochemistry · 2022Article
- Article
- Skeletal muscle DNA methylation modifications and psychopharmacologic treatment in bipolar disorder.European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology · 2019Article
- DNA Methylation Validation Methods: a Coherent Review with Practical Comparison.Biological procedures online · 2019Review
- The prevalence and persistence of aberrant promoter DNA methylation in benzene-exposed Chinese workers.PloS one · 2019Article
- TFF1 Promotes EMT-Like Changes through an Auto-Induction Mechanism.International journal of molecular sciences · 2018Article
- Long-term Persistent Organic Pollutants Exposure Induced Telomere Dysfunction and Senescence-Associated Secretary Phenotype.The journals of gerontology. Series A, Biological sciences and medical sciences · 2018Article
- Association of Protein Kinase B (AKT) DNA Hypermethylation with Maintenance Atypical Antipsychotic Treatment in Patients with Bipolar Disorder.Pharmacotherapy · 2018Observational
- Identification of reliable biomarkers of human papillomavirus 16 methylation in cervical lesions based on integration status using high-resolution melting analysis.Clinical epigenetics · 2018Article
- DNA Methyltransferase 1-Dependent DNA Hypermethylation Constrains Arteriogenesis by Augmenting Shear Stress Set Point.Journal of the American Heart Association · 2017Article
- Forensic age prediction for saliva samples using methylation-sensitive high resolution melting: exploratory application for cigarette butts.Scientific reports · 2017Article
- Chiba study of Mother and Children's Health (C-MACH): cohort study with omics analyses.BMJ open · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe methylation of DNA is recognized as a key mechanism in the regulation of genomic stability and evidence for its role in the development of cancer is accumulating. LINE-1 methylation status represents a surrogate measure of genome-wide methylation.
findingsUsing high resolution melt (HRM) curve analysis technology, we have established an in-tube assay that is linear (r > 0.9986) with a high amplification efficiency (90-105%), capable of discriminating between partcipant samples with small differences in methylation, and suitable for quantifying a wide range of LINE-1 methylation levels (0-100%)--including the biologically relevant range of 50-90% expected in human DNA. We have optimized this procedure to perform using 2 μg of starting DNA and 2 ng of bisulfite-converted DNA for each PCR reaction. Intra- and inter-assay coefficients of variation were 1.44% and 0.49%, respectively, supporting the high reproducibility and precision of this approach.
conclusionsIn summary, this is a completely linear, quantitative HRM PCR method developed for the measurement of LINE-1 methylation. This cost-efficient, refined and reproducible assay can be performed using minimal amounts of starting DNA. These features make our assay suitable for high throughput analysis of multiple samples from large population-based studies.
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