Evidence map›Paper›PMID 22204564›Full record

ArticleLangmuir : the ACS journal of surfaces and colloids2012

Multiprotein microcontact printing with micrometer resolution.

Colin D Eichinger, Tony W Hsiao, Vladimir Hlady

Abstract read
In one paragraph

Article in Langmuir : the ACS journal of surfaces and colloids, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Colin D EichingerDepartment of Bioengineering, University of Utah, Salt Lake City, Utah 84112, USA.
Tony W Hsiao
Vladimir Hlady
University of Utah · US

Funding

NEURONAL PATHFINDING ON MODIFIED PROTEOGLYCANSR01NS057144 · NINDS · UNIVERSITY OF UTAH · PI HLADY, VLADIMIR · 2008 to 2011
$1.7M
NINDS NIH HHS 5R01NS57144NINDS NIH HHS R01 NS057144
6 · The paper itself

Abstract

Depositing multiple proteins on the same substrate in positions similar to the natural cellular environment is essential to tissue engineering and regenerative medicine. In this study, the development and verification of a multiprotein microcontact printing (μCP) technique is described. It is shown that patterns of multiple proteins can be created by the sequential printing of proteins with micrometer precision in registration using an inverted microscope. Soft polymeric stamps were fabricated and mounted on a microscope stage while the substrate to be stamped was placed on a microscope objective and kept at its focal distance. This geometry allowed for visualization of patterns during the multiple stamping events and facilitated the alignment of multiple stamped patterns. Astrocytes were cultured over stamped lane patterns and were seen to interact and align with the underlying protein patterns.

Indexed as

ProteinsAggrecansAnimalsAstrocytesCell AdhesionLamininMicroscopyMicrotechnologyOptical PhenomenaPrintingRatsSoftwareAggrecansLamininProteins

Identifiers

PMID22204564
PMCPMC3269504
OpenAlexW2334964234

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.