ArticleBMC cancer2011
Baicalein mediates inhibition of migration and invasiveness of skin carcinoma through Ezrin in A431 cells.
Article in BMC cancer, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 33 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Fish consumption and gastric cancer within the Stomach cancer Pooling (StoP) Project.Scientific reports · 2025Pooled it
- p-Coumaric acid inhibits hepatocellular carcinoma through promoting ROS accumulation.Medical oncology (Northwood, London, England) · 2026Article
- Antioxidant and Antiproliferation Activities of Lemon Verbena (Plants (Basel, Switzerland) · 2022Article
- An overview of pharmacological activities of baicalin and its aglycone baicalein: New insights into molecular mechanisms and signaling pathways.Iranian journal of basic medical sciences · 2022Review
- NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation.Nature cancer · 2021Article
- Baicalein inhibits non-small-cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment.Cancer science · 2020Article
- Immunohistochemical Study of Ezrin Expression in Colorectal Carcinoma: A Comparative Study between Objective Method and Digital Quantitative Assessment.Asian Pacific journal of cancer prevention : APJCP · 2020Article
- Ezrin Mediates Invasion and Metastasis in Tumorigenesis: A Review.Frontiers in cell and developmental biology · 2020Review
- Fighting Thyroid Cancer with Microgravity Research.International journal of molecular sciences · 2019Review
- Emerging Roles of the Endoplasmic Reticulum Associated Unfolded Protein Response in Cancer Cell Migration and Invasion.Cancers · 2019Review
- Article
- microRNA-211 regulates cell proliferation, apoptosis and migration/invasion in human osteosarcoma via targeting EZRIN.Cellular & molecular biology letters · 2019Article
- Baicalein induced apoptosis and autophagy of undifferentiated thyroid cancer cells by the ERK/PI3K/Akt pathway.American journal of translational research · 2019Article
- Effects of Prisma® Skin dermal regeneration device containing glycosaminoglycans on human keratinocytes and fibroblasts.Cell adhesion & migration · 2018Article
- Molecular background of the regional lymph node metastasis of gastric cancer.Oncology letters · 2018Review
- Anticancer Effects of Baicalein in Pancreatic Neuroendocrine Tumors In Vitro and In Vivo.Pancreas · 2017Article
- Baicalein Inhibits Epithelial to Mesenchymal Transition via Downregulation of Cyr61 and LOXL-2 in MDA-MB231 Breast Cancer Cells.Molecules and cells · 2016Article
- Cyclic Glucans Enhance Solubility of Bioavailable Flavonoids.Molecules (Basel, Switzerland) · 2016Review
- The Fascinating Effects of Baicalein on Cancer: A Review.International journal of molecular sciences · 2016Review
- Anticancer properties of baicalein: a review.Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents · 2016Article
Corrections and comments
- Retraction · 2022-03-15Duplication of/in Image · Unreliable Data · · see also: https://pubpeer.com/publications/0CA68D7B009E65B9DD252F8A932B98
- Retracted
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEzrin is highly expressed in skin cancer and promotes tumor metastasis. Ezrin serves as a promising target for anti-metastasis therapy. The aim of this study is to determine if the flavonoid bacailein inhibits the metastasis of skin cancer cells through Ezrin.
methodsCells from a cutaneous squamous carcinoma cell line, A431, were treated with baicalein at 0-60 μM to establish the non-cytotoxic concentration (NCC) range for baicalein. Following treatment with baicalein within this range, total Ezrin protein (both phosphorylated and unphosphorylated forms) and phosphorylated-Ezrin (phos-Ezrin) were detected by western blotting, and Ezrin RNA was detected in A431 cells using reverse transcription-polymerase chain reaction (RT-PCR). Thereafter, the motility and invasiveness of A431 cells following baicalein treatment were determined using wound-healing and Boyden chamber invasion assays. Short-interfering RNA (si-RNA) specifically targeting Ezrin was transfected into A431 cells, and a si-RNA Ezrin-A431 cell line was established by G418 selection. This stable cell line was transiently transfected with Ezrin and mutant Ezrin plasmids, and its motility and invasiveness was subsequently determined to clarify whether bacailein inhibits these processes through Ezrin.
resultsWe determined the range of NCCs for baicalein to be 2.5-40 μM in A431 cells. Baicalein displayed a dose- and time-dependent inhibition of expressions of total Ezrin and phos-Ezrin within this range NCCs. In addition, it exerted this inhibitory effect through the reduction of Ezrin RNA transcript. Baicalein also inhibited the motility and invasiveness of A431 skin carcinoma cells within the range of NCCs, in a dose- and time-dependent manner. A431 cell motility and invasiveness were inhibited by 73% and 80% respectively when cells were treated with 20 μM baicalein. However, the motility and invasiveness of A431 cells containing the Ezrin mutant were not effectively inhibited by baicalein.
conclusionsBaicalein reduces the migration and invasiveness of A431 cells through the inhibition of Ezrin expression, which leads to the suppression of tumor metastasis.
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