ArticleProceedings of the National Academy of Sciences of the United States of America2012
Sperm-borne microRNA-34c is required for the first cleavage division in mouse.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 202 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
202 citing papers in PubMed, 1 synthesis or guideline pooled it, 459 citations in OpenAlex.
- Qualitative and quantitative assessment of sperm miRNAs identifies hsa-miR-9-3p, hsa-miR-30b-5p and hsa-miR-122-5p as potential biomarkers of male infertility and sperm quality.Reproductive biology and endocrinology : RB&E · 2022Pooled it
- Seminal miRNA Relationship with Apoptotic Markers and Oxidative Stress in Infertile Men with Varicocele.BioMed research international · 2016Trial
- Doxorubicin and 4-Hydroperoxycyclophosphamide Alter Mitochondrial Dynamics and miR-34 Expression in Spermatogonia and Spermatocytes In Vitro.Basic & clinical pharmacology & toxicology · 2026Article
- Heat Stress and the Bull Sperm Transcriptome: Molecular Mechanisms, Fertility, and Epigenetic Inheritance.Animals : an open access journal from MDPI · 2026Review
- MicroRNAs as Regulators of Reproductive Function: From Gametogenesis to Pregnancy.Journal of developmental biology · 2026Review
- In Vitro Sperm-Epididymosomes Interaction Immediately Before Fertilization Changes Sperm Fertility Potential.Andrology · 2026Article
- Sperm 28S ribosomal RNA fragments as a potential biomarker for embryo quality in in vitro fertilization.Journal of assisted reproduction and genetics · 2026Article
- Epididymosome-Supplemented Extender Induces Changes in Morpho-Functional Traits and microRNA Levels of Post-Thaw Sperm and Improves Embryo Developmental Potential.Molecular reproduction and development · 2026Article
- Small RNA profile alterations as a potential mechanism for sperm quality improvement following varicocelectomy.Asian journal of andrology · 2026Article
- Transgenerational Epigenetic Effect of Cryopreservation of F0 Rooster Sperm (Animals : an open access journal from MDPI · 2026Article
- From sperm to pregnancy: insights into paternal regulation of fertility divergence in cattle.The Journal of reproduction and development · 2026Review
- Low expression of hsa-miR-34c-5p in sperm is associated with unexplained recurrent miscarriage.Frontiers in genetics · 2026Article
- Gene regulation by non-Coding RNAs in infertility: a mechanistic review.Journal of ovarian research · 2025Review
- Exposure of mice to environmentally relevant per- and polyfluoroalkyl substances (PFAS) alters the sperm epigenome.Communications biology · 2025Article
- Sperm abnormality: Differential expression of microRNAs.Journal of assisted reproduction and genetics · 2025Review
- Age-related decline in behavior and reproductive health in male mice.Scientific reports · 2025Article
- MicroRNA-34a-5p induces cell cycle arrest and leads to spermatogenesis failure by targeting CDC25A.Cellular and molecular life sciences : CMLS · 2025Article
- Exploration of Small Non-Coding RNAs as Molecular Markers of Ram Sperm Fertility.International journal of molecular sciences · 2025Article
- The composition of human sperm sncRNAome: a cross-country small RNA profiling.Reproductive biology and endocrinology : RB&E · 2025Article
- Paternal Effects in Mammalian Reproduction: Functional, Environmental, and Clinical Relevance of Sperm Components in Early Embryos and Beyond.Molecular reproduction and development · 2025Review
142 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammals, the sperm deliver mRNA of unknown function into the oocytes during fertilization. The role of sperm microRNAs (miRNAs) in preimplantation development is unknown. miRNA profiling identified six miRNAs expressed in the sperm and the zygotes but not in the oocytes or preimplantation embryos. Sperm contained both the precursor and the mature form of one of these miRNAs, miR-34c. The absence of an increased level of miR-34c in zygotes derived from α-amanitin-treated oocytes and in parthenogenetic oocytes supported a sperm origin of zygotic miR-34c. Injection of miR-34c inhibitor into zygotes inhibited DNA synthesis and significantly suppressed first cleavage division. A 3' UTR luciferase assay and Western blotting demonstrated that miR-34c regulates B-cell leukemia/lymphoma 2 (Bcl-2) expression in the zygotes. Coinjection of anti-Bcl-2 antibody in zygotes partially reversed but injection of Bcl-2 protein mimicked the effect of miR-34c inhibition. Oocyte activation is essential for the miR-34c action in zygotes, as demonstrated by a decrease in 3'UTR luciferase reporter activity and Bcl-2 expression after injection of precursor miR-34c into parthenogenetic oocytes. Our findings provide evidence that sperm-borne miR-34c is important for the first cell division via modulation of Bcl-2 expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.