ArticleMolecular biology reports2012
Evaluation of suitable reference genes for quantitative RT-PCR during development and abiotic stress in Panonychus citri (McGregor) (Acari: Tetranychidae).
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Winter-active spiders (Clubiona) have a hyperactive antifreeze protein with a unique beta-solenoid fold.The FEBS journal · 2026Article
- Screening and Identification of Reference Genes forInsects · 2025Article
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- Transcriptome analysis of the spiderThe journal of venomous animals and toxins including tropical diseases · 2023Article
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- Reference Gene Selection for Analyzing the Transcription Patterns of Two Fatty Acyl-CoA Reductase Genes From Paracoccus marginatus (Hemiptera: Pseudococcidae).Journal of insect science (Online) · 2021Article
- Reference gene and tropomyosin expression in mud crab Scylla olivacea, Scylla paramamosain and Scylla tranquebarica.Molecular biology reports · 2020Article
- Reference gene validation in Eotetranychus sexmaculatus (Acari: Tetranychidae) feeding on mite-susceptible and mite-resistant rubber tree germplasms.Experimental & applied acarology · 2020Article
- Selection of Reference Genes for RT-qPCR Analysis Under Extrinsic Conditions in the Hawthorn Spider Mite,Frontiers in physiology · 2020Article
- A Shift Pattern of Bacterial Communities Across the Life Stages of the Citrus Red Mite,Frontiers in microbiology · 2020Article
- Reference genes for gene expression studies by RT-qPCR in Brevipalpus yothersi (Acari: Tenuipalpidae), the mite vector of citrus leprosis virus.Scientific reports · 2019Article
- Selection of Reference Genes for RT-qPCR Analysis Under Intrinsic Conditions in the Hawthorn Spider Mite,Frontiers in physiology · 2019Article
- Biofunctional analysis of Vitellogenin and Vitellogenin receptor in citrus red mites, Panonychus citri by RNA interference.Scientific reports · 2017Article
- Abamectin treatment affects glutamate decarboxylase expression and induces higher GABA levels in the citrus red mite, Panonychus citri.Experimental & applied acarology · 2017Article
- Article
- Photo-enzymatic repair of UVB-induced DNA damage in the two-spotted spider mite Tetranychus urticae.Experimental & applied acarology · 2017Article
- Functional analysis of a chitinase gene during the larval-nymph transition in Panonychus citri by RNA interference.Experimental & applied acarology · 2016Article
- Selection of Reference Genes for Expression Studies of Xenobiotic Adaptation in Tetranychus urticae.International journal of biological sciences · 2016Article
- Article
- Regulation of three isoforms of SOD gene by environmental stresses in citrus red mite, Panonychus citri.Experimental & applied acarology · 2015Article
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Quantitative real time reverse transcriptase polymerase chain reaction (RT-qPCR) is preferred for gene expression analysis in living organisms. Currently, it is a valuable tool for biological and ecological studies as it provides a relatively straightforward way to assess the relevance of transcriptional regulation under developmental and stress tolerance conditions. However, studies have shown that some commonly used reference genes varied among different experimental treatments, thus, systematic evaluation of reference genes is critical for gene expression profiling, which is often neglected in gene expression studies of arthropods. The aim of this study is to identify the suitable reference genes for RT-qPCR experiments involving various developmental stages and/or under abiotic stresses in citrus red mite Panonychus citri, a key pest in citrus orchards worldwide. GeNorm, NormFinder, and Bestkeeper software analysis indicates that elongation factor-1 alpha (ELF1A), RNA polymerase II largest subunit, alpha tublin, and glyceraldhyde-3-phosphate dehydrogenase (GAPDH) are the most stable reference genes in various developmental stages, meanwhile, ELF1A and GAPDH were the most stable reference genes under various abiotic stresses. Furthermore, this study will serve as a resource to screen reference genes for gene expression studies in any other spider mite species.
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