Evidence map›Paper›PMID 22203323›Full record

ArticlePlanta2012

Molecular characterization of a new waxy allele with partial expression in spelt wheat.

Carlos Guzmán, Leonor Caballero, Makoto Yamamori, Juan B Alvarez

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Research Progress on the Regulatory Mechanism of theCurrent issues in molecular biology · 2025
    Review
  3. Article
  4. Review
  5. Review
  6. Interspecific and intergeneric hybridization as a source of variation for wheat grain quality improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018
    Review
  7. Transposon insertion resulted in the silencing of Wx-B1n in Chinese wheat landraces.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017
    Article
  8. Wheat waxy proteins: polymorphism, molecular characterization and effects on starch properties.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Carlos GuzmánDepartamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain. ge2gugac@uco.es
Leonor Caballero
Makoto Yamamori
Juan B Alvarez
University of Córdoba · ESInstitute of Crop Science · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Starch composition which is dependent on the waxy protein, the enzyme responsible for amylose synthesis in the grain, is an important aspect of the wheat quality. In this report, we describe the characterization of a novel Wx-A1 allele (Wx-A1g formerly known as -Wx-A1a) in Spanish spelt wheat lines which is responsible for a remarkable decline in the concentration of Wx-A1 protein found in the endosperm. Comparison of the DNA sequences in the Wx-A1a and Wx-A1g alleles showed the presence of a 160-bp insertion within the fourth intron in the latter. This insertion had some characteristics of a transposable-like element. RT-PCR analysis showed the presence of normal and aberrant mRNA transcripts in the Wx-A1g lines, indicating that the aberrant transcripts are un-spliced and contained the longer fourth intron. This may be related to the low level of Wx-A1 protein in these lines. In addition, a simple and fast PCR assay was designed for differentiating among different Wx-A1 alleles (a, b, f and g). The mutation described here is not related to either of the Wx-A1 mutations identified previously in common and durum wheats and could help to extend the range of amylose content of wheats.

Indexed as

AllelesGene Expression Regulation, PlantBase SequenceElectrophoresis, Gel, Two-DimensionalGenes, PlantGenetic MarkersMolecular Sequence DataMutagenesis, InsertionalPlant ProteinsReverse Transcriptase Polymerase Chain ReactionStarch SynthaseTriticumGenetic MarkersPlant ProteinsStarch Synthasewaxy protein, plant

Identifiers

PMID22203323
OpenAlexW1977225995

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.