ArticleMolecular genetics and genomics : MGG2012
Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.
Article in Molecular genetics and genomics : MGG, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 110 citations in OpenAlex.
- Genome-wide identification and gene expression analysis of Formin homology 2 (FH2) genes in peanut.BMC plant biology · 2026Article
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- Genome-wide identification of peanut ERFs and functional characterization of AhERF28 in response to salt and drought stresses.Plant cell reports · 2025Article
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- Genome-Wide Identification of PeanutPlants (Basel, Switzerland) · 2024Article
- Identification and selection of reference genes for analysis of gene expression by quantitative real-time PCR in the euhalophyteCommunicative & integrative biology · 2024Article
- Fine mapping of qAHPS07 and functional studies of AhRUVBL2 controlling pod size in peanut (Arachis hypogaea L.).Plant biotechnology journal · 2023Article
- Molecular cloning and functional characterization of the promoter of a novelFrontiers in plant science · 2023Article
- Genome-wide characterization of phospholipase D family genes in allotetraploid peanut and its diploid progenitors revealed their crucial roles in growth and abiotic stress responses.Frontiers in plant science · 2023Article
- A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenicFrontiers in plant science · 2023Article
- Genome-Wide Characterization of Ascorbate Peroxidase Gene Family in Peanut (Frontiers in plant science · 2022Article
- Integrated analyses of transcriptome and metabolome provides new insights into the primary and secondary metabolism in response to nitrogen deficiency and soil compaction stress in peanut roots.Frontiers in plant science · 2022Article
- Identification and selection of reference genes for gene expression analysis by quantitative real-time PCR in Suaeda glauca's response to salinity.Scientific reports · 2021Article
- Spatio-temporal selection of reference genes in the two congeneric species of Glycyrrhiza.Scientific reports · 2021Article
- Transcriptome and Metabolome Analysis Unveil Anthocyanin Metabolism in Pink and Red Testa of Peanut (International journal of genomics · 2021Article
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- Cloning and Functional Characterization of a Pericarp Abundant Expression Promoter (Frontiers in genetics · 2021Article
- Selection of Reference Genes for qRT-PCR Analysis in Medicinal PlantPlants (Basel, Switzerland) · 2020Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time reverse transcription PCR (qRT-PCR), a sensitive technique for quantifying gene expression, depends on the stability of the reference gene(s) used for data normalization. Only a few studies on the reference genes have been done with peanut to date. In the present study, 14 potential reference genes in peanut were evaluated for their expression stability using the geNorm and NormFinder statistical algorithms. Expression stability was assessed by qRT-PCR across 32 biological samples, including various tissue types, seed developmental stages, salt and cold treatments. The results showed that the best-ranked references genes differed across the samples. UKN1, UKN2, TUA5 and ACT11 were the most stable across all the tested samples. A combination of ACT11, TUA5, UKN2, PEPKR1 and TIP41 would be appropriate as a reference panel for normalizing gene expression data across the various tissues tested, whereas the combination of TUA5 and UKN1 was the most suitable for seed developmental stages. TUA5 and EF1b exhibited the most stable expression under cold treatment. For salt-treated leaves, TUA5 and UKN2 were the most stably expressed and HDC and UKN1 for salt-treated roots. The relative gene expression level of peanut Cys(2)/His(2)-type zinc finger protein gene AhZFP1 was analyzed in order to validate the reference genes selected for this study. These results provide guidelines for the selection of reference genes under different experimental conditions and also a foundation for more accurate and widespread use of qRT-PCR in peanut gene analysis.
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