ArticleMolecular and cellular biology2012
AU-rich-element-dependent translation repression requires the cooperation of tristetraprolin and RCK/P54.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 74 citations in OpenAlex.
- Reorganization of Cell Compartmentalization Induced by Stress.Biomolecules · 2022Review
- RNA Binding Protein OsTZF7 Traffics Between the Nucleus and Processing Bodies/Stress Granules and Positively Regulates Drought Stress in Rice.Frontiers in plant science · 2022Article
- Posttranscriptional regulation of ILC2 homeostatic function via tristetraprolin.The Journal of experimental medicine · 2021Article
- The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain.Journal of inflammation (London, England) · 2021Article
- RNA decay in processing bodies is indispensable for adipogenesis.Cell death & disease · 2021Article
- Zinc finger protein ZFP36L1 inhibits influenza A virus through translational repression by targeting HA, M and NS RNA transcripts.Nucleic acids research · 2020Article
- Interactions among glomerulus infiltrating macrophages and intrinsic cells via cytokines in chronic lupus glomerulonephritis.Journal of autoimmunity · 2020Article
- The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis.Cell stem cell · 2019Article
- Molecular basis for GIGYF-Me31B complex assembly in 4EHP-mediated translational repression.Genes & development · 2019Article
- Overview of RAW264.7 for osteoclastogensis study: Phenotype and stimuli.Journal of cellular and molecular medicine · 2019Review
- Quantification and discovery of sequence determinants of protein-per-mRNA amount in 29 human tissues.Molecular systems biology · 2019Article
- Dissecting mRNA decay and translation inhibition during iron deficiency.Current genetics · 2019Review
- Yeast Cth2 protein represses the translation of ARE-containing mRNAs in response to iron deficiency.PLoS genetics · 2018Article
- Article
- P38 activation induces the dissociation of tristetraprolin from Argonaute 2 to increase ARE-mRNA stabilization.Molecular biology of the cell · 2018Article
- Uncovering the Role of RNA-Binding Proteins in Gene Expression in the Immune System.Frontiers in immunology · 2018Review
- Targeting RNA helicases in cancer: The translation trap.Biochimica et biophysica acta. Reviews on cancer · 2017Review
- Article
- The RNA-binding protein Tristetraprolin (TTP) is a critical negative regulator of the NLRP3 inflammasome.The Journal of biological chemistry · 2017Article
- Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
15 authors at 6 institutions in 2 countries.
Funding
Abstract
AU-rich elements (AREs), residing in the 3' untranslated region (UTR) of many labile mRNAs, are important cis-acting elements that modulate the stability of these mRNAs by collaborating with trans-acting factors such as tristetraprolin (TTP). AREs also regulate translation, but the underlying mechanism is not fully understood. Here we examined the function and mechanism of TTP in ARE-mRNA translation. Through a luciferase-based reporter system, we used knockdown, overexpression, and tethering assays in 293T cells to demonstrate that TTP represses ARE reporter mRNA translation. Polyribosome fractionation experiments showed that TTP shifts target mRNAs to lighter fractions. In murine RAW264.7 macrophages, knocking down TTP produces significantly more tumor necrosis factor alpha (TNF-α) than the control, while the corresponding mRNA level has a marginal change. Furthermore, knockdown of TTP increases the rate of biosynthesis of TNF-α, suggesting that TTP can exert effects at translational levels. Finally, we demonstrate that the general translational repressor RCK may cooperate with TTP to regulate ARE-mRNA translation. Collectively, our studies reveal a novel function of TTP in repressing ARE-mRNA translation and that RCK is a functional partner of TTP in promoting TTP-mediated translational repression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.