ArticleInternational journal of molecular epidemiology and genetics2011
Epidemiologic Investigation of Extra-intestinal pathogenic E. coli (ExPEC) based on PCR phylogenetic group and fimH single nucleotide polymorphisms (SNPs) in China.
Article in International journal of molecular epidemiology and genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
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- Role for FimH in Extraintestinal Pathogenic Escherichia coli Invasion and Translocation through the Intestinal Epithelium.Infection and immunity · 2017Article
- Foley catheters functionalised with a synergistic combination of antibiotics and silver nanoparticles resist biofilm formation.IET nanobiotechnology · 2017Article
- Trends in ExPEC serogroups in the UK and their significance.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Discovery of novel xylosides in co-culture of basidiomycetes Trametes versicolor and Ganoderma applanatum by integrated metabolomics and bioinformatics.Scientific reports · 2016Article
- Occurrence of genes encoding enterotoxins in uropathogenic Escherichia coli isolates.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2015Article
- Comparative virulence of urinary and bloodstream isolates of extra-intestinal pathogenic Escherichia coli in a Galleria mellonella model.Virulence · 2015Article
- Genomic characterisation of an endometrial pathogenic Escherichia coli strain reveals the acquisition of genetic elements associated with extra-intestinal pathogenicity.BMC genomics · 2014Article
- The high prevalence of serine protease autotransporters of Enterobacteriaceae (SPATEs) in Escherichia coli causing neonatal septicemia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
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- Point mutations in FimH adhesin of Crohn's disease-associated adherent-invasive Escherichia coli enhance intestinal inflammatory response.PLoS pathogens · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using the combination method with PCR phylogrouping and fimH SNPs analysis, this study investigates the epidemiology of Extra-intestinal pathogenic E. coli in China. 116 E. coli strains including (74 from Urine, 39 from other extra-intestinal sources and 3 references strains) were collected. The bacteria Genomic DNA were extracted; phylogroup and the fimH gene amplifications were determined by two-step triplex PCR-based phylogrouping and simple PCR amplification assay respectively. Finally the fimH SNPs analysis and phylogenetic analysis and construction of tree were carried out using DNAMAN Version 6.0.3.93 and MEGA4, ClustalW and CLC Bio software respectively for 50 E. coli strains isolated from clinical sample and 3 references; K-12 E. coli strain was used as reference comparison. For E. coli strains phylogroup, 25% (28/113) were observed to belong to the group A, 15% (17/113) to the group B1, 14% (16/113) to the group B2, and 46% (52/113) to the group D. 75% (85/113) were fimH positive. fimH SNPs analysis for 50 isolated from clinical sample and 3 references found 60 SNPs at 57 polymorphic sites. The number of amino-acid variants and silent SNPs were observed more in UPEC strains than in other extra-intestinal E. coli strains. Most of the UPEC strains with the same amino-acid variants were belong to the same phylogroup. This combination method could serve as a rapid, highly reproducible typing test for epidemiological studies of ExPEC. Large collection data could be compared with other clinical laboratories that the sequence data are accessible.
Indexed as
Identifiers
22199997PMC3243450W2155682773What OpenQuestion holds
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