ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2012
Noninvasive detection of neural progenitor cells in living brains by MRI.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Lipopolysaccharide Induced Opening of the Blood Brain Barrier on Aging 5XFAD Mouse Model.Journal of Alzheimer's disease : JAD · 2019Article
- Detection of Stem Cell Transplant Rejection with Ferumoxytol MR Imaging: Correlation of MR Imaging Findings with Those at Intravital Microscopy.Radiology · 2017Article
- Epigenetics of amphetamine-induced sensitization: HDAC5 expression and microRNA in neural remodeling.Journal of biomedical science · 2016Article
- Amphetamine manipulates monoamine oxidase-A level and behavior using theranostic aptamers of transcription factors AP-1/NF-kB.Journal of biomedical science · 2016Article
- Imaging rhodopsin degeneration in vivo in a new model of ocular ischemia in living mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Article
- Anatomical, functional and molecular biomarker applications of magnetic resonance neuroimaging.Future neurology · 2015Article
- Uptake and metabolism of iron oxide nanoparticles in brain cells.Neurochemical research · 2014Review
- New applications of nanotechnology for neuroimaging.AJNR. American journal of neuroradiology · 2014Article
- Intracellular gene transcription factor protein-guided MRI by DNA aptamers in vivo.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Emerging methods for disease monitoring in malignant gliomas.CNS oncology · 2013Review
- MRI reveals differential effects of amphetamine exposure on neuroglia in vivo.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Bioengineered probes for molecular magnetic resonance imaging in the nervous system.ACS chemical neuroscience · 2012Review
- Is there a path beyond BOLD? Molecular imaging of brain function.NeuroImage · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The presence of pericytes in brain regions undergoing repair is evident of the recruitment of bone marrow-derived multipotent regenerative cells to the neurovascular unit during angiogenesis. At present, post mortem sampling is the only way to identify them. Therefore, such cell typing is inadequate for preserving neural progenitor cells for any meaningful stem cell therapy. We aimed to target cerebral pericytes in vivo using dual gene transcript-targeted MRI (GT-tMRI) in male C57black6 mice after a 60-min bilateral carotid artery occlusion (BCAO). We attached superparamagnetic iron oxide nanoparticles (SPIONs) to phosphorothioate-modified micro-DNA that targets actin or nestin mRNA. Because BCAO compromises the blood-brain barrier (BBB) and induces expression of α-smooth muscle (αSM)-actin and nestin antigens by pericytes in new vessels, we delivered pericyte-specific magnetic resonance contrast agents (SPION-actin or SPION-nestin at 4 mg Fe/kg) by i.p. injection to C57black6 mice that had experienced BCAO. We demonstrated that the surge in cerebral iron content by inductively coupled plasma-mass spectrometry matched the increase in the frequency of relaxivity. We also found that SPION-nestin was colocalized in αSM- actin- and nestin-expressing pericytes in BCAO-treated C57black6 or transgenic mice [B6.Cg-Tg(CAG-mRFP1) 1F1Hadj/J, expressing red fluorescent protein by actin promoter]. We identified pericytes in the repair patch in living brains after BCAO with a voxel size of 0.03 mm(3). The presence of electron-dense nanoparticles in vascular pericytes in the region of BBB injury led us to draw the conclusion that GT-tMRI can noninvasively reveal neural progenitor cells during vascularization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.