ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2012
A mutation screening platform for rapeseed (Brassica napus L.) and the detection of sinapine biosynthesis mutants.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Joint analysis of GWAS and TILLING reveals the negative role of GLABRA2 in controlling seed oil content in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Genomic variation induced by a low concentration of ethyl methanesulfonate (EMS) in quinoa 'Longli-4' variety.Botanical studies · 2024Article
- Knockout ofFrontiers in plant science · 2024Article
- Breeding and biotechnology approaches to enhance the nutritional quality of rapeseed byproducts for sustainable alternative protein sources- a critical review.Frontiers in plant science · 2024Review
- Efficient multi-allelic genome editing via CRISPR-Cas9 ribonucleoprotein-based delivery toFrontiers in plant science · 2024Article
- Article
- Reduced glucosinolate content in oilseed rape (Brassica napus L.) by random mutagenesis of BnMYB28 and BnCYP79F1 genes.Scientific reports · 2023Article
- Is it the end of TILLING era in plant science?Frontiers in plant science · 2023Review
- Perspectives for integrated insect pest protection in oilseed rape breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Review
- Diverting phenylpropanoid pathway flux from sinapine to produce industrially useful 4-vinyl derivatives of hydroxycinnamic acids in Brassicaceous oilseeds.Metabolic engineering · 2022Article
- Breeding Canola (Plants (Basel, Switzerland) · 2021Review
- Genomic background selection to reduce the mutation load after random mutagenesis.Scientific reports · 2021Article
- Genomics Armed With Diversity Leads the Way inFrontiers in genetics · 2021Review
- Loss of function of CRT1a (calreticulin) reduces plant susceptibility to Verticillium longisporum in both Arabidopsis thaliana and oilseed rape (Brassica napus).Plant biotechnology journal · 2020Article
- Molecular and genetic bases of heat stress responses in crop plants and breeding for increased resilience and productivity.Journal of experimental botany · 2020Review
- Knockout ofFrontiers in plant science · 2020Article
- A missense mutation of STERILE APETALA leads to female sterility in Chinese cabbage (Brassica campestris ssp. pekinensis).Plant reproduction · 2019Article
- An APETALA1 ortholog affects plant architecture and seed yield component in oilseed rape (Brassica napus L.).BMC plant biology · 2018Article
- The effect of INDEHISCENT point mutations on silique shatter resistance in oilseed rape (Brassica napus).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- The Pattern and Distribution of Induced Mutations inFrontiers in plant science · 2018Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed two mutant populations of oilseed rape (Brassica napus L.) using EMS (ethylmethanesulfonate) as a mutagen. The populations were derived from the spring type line YN01-429 and the winter type cultivar Express 617 encompassing 5,361 and 3,488 M(2) plants, respectively. A high-throughput screening protocol was established based on a two-dimensional 8× pooling strategy. Genes of the sinapine biosynthesis pathway were chosen for determining the mutation frequencies and for creating novel genetic variation for rapeseed breeding. The extraction meal of oilseed rape is a rich protein source containing about 40% protein. Its use as an animal feed or human food, however, is limited by antinutritive compounds like sinapine. The targeting-induced local lesions in genomes (TILLING) strategy was applied to identify mutations of major genes of the sinapine biosynthesis pathway. We constructed locus-specific primers for several TILLING amplicons of two sinapine synthesis genes, BnaX.SGT and BnaX.REF1, covering 80-90% of the coding sequences. Screening of both populations revealed 229 and 341 mutations within the BnaX.SGT sequences (135 missense and 13 nonsense mutations) and the BnaX.REF1 sequences (162 missense, 3 nonsense, 8 splice site mutations), respectively. These mutants provide a new resource for breeding low-sinapine oilseed rape. The frequencies of missense and nonsense mutations corresponded to the frequencies of the target codons. Mutation frequencies ranged from 1/12 to 1/22 kb for the Express 617 population and from 1/27 to 1/60 kb for the YN01-429 population. Our TILLING resource is publicly available. Due to the high mutation frequencies in combination with an 8× pooling strategy, mutants can be routinely identified in a cost-efficient manner. However, primers have to be carefully designed to amplify single sequences from the polyploid rapeseed genome.
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