ArticleJournal of experimental botany2012
Isolation and characterization of galactinol synthases from hybrid poplar.
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Tonoplast sucrose transporter SUT4-dependent sugar partitioning modulates phenological transitions and reproductive success in poplar.The Plant journal : for cell and molecular biology · 2025Article
- Article
- Optimizing raffinose family oligosaccharides content in plants: A tightrope walk.Frontiers in plant science · 2023Review
- Mobile forms of carbon in trees: metabolism and transport.Tree physiology · 2022Review
- Stem girdling affects the onset of autumn senescence in aspen in interaction with metabolic signals.Physiologia plantarum · 2021Article
- Article
- Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy.Journal of experimental botany · 2018Article
- Genome-Wide Identification, Evolutionary and Expression Analyses of the GALACTINOL SYNTHASE Gene Family in Rapeseed and Tobacco.International journal of molecular sciences · 2017Article
- Suppression of cucumber stachyose synthase gene (CsSTS) inhibits phloem loading and reduces low temperature stress tolerance.Plant molecular biology · 2017Article
- Altering carbon allocation in hybrid poplar (Populus alba × grandidentata) impacts cell wall growth and development.Plant biotechnology journal · 2017Article
- Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus.Scientific reports · 2016Article
- Genome-wide transcriptomic and proteomic analyses of bollworm-infested developing cotton bolls revealed the genes and pathways involved in the insect pest defence mechanism.Plant biotechnology journal · 2016Article
- Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season.Plant molecular biology · 2015Article
- Significance of galactinol and raffinose family oligosaccharide synthesis in plants.Frontiers in plant science · 2015Review
- Article
- Responses of Populus trichocarpa galactinol synthase genes to abiotic stresses.Journal of plant research · 2014Article
- Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato.Journal of experimental botany · 2013Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The raffinose family of oligosaccharides (RFOs) serve as transport carbohydrates in the phloem, storage compounds in sink tissues, and putative biological agents to combat both abiotic and biotic stress in several plant species. To investigate further the functional roles of this class of compounds in trees, two cDNAs encoding galactinol synthase (GolS, EC 2.4.1.123), which catalyses the first step in the biosynthesis of RFOs, were identified and cloned from hybrid poplar (Populus alba×grandidentata). Phylogenetic analyses of the Populus GolS isoforms with other known GolS proteins suggested a putative role for these enzymes during biotic or abiotic stress in hybrid poplar. The predicted protein sequences of both isoforms (Pa×gGolSI and Pa×gGolSII) showed characteristics of GolS proteins from other species, including a serine phosphorylation site and the ASAAP pentapeptide hydrophobic domain. Kinetic analyses of recombinant Pa×gGolSI and Pa×gGolSII resulted in K(m) values for UPD-galactose of 0.80 and 0.65 mM and V(max) values of 657.5 and 1245 nM min(-1), respectively. Pa×gGolSI inherently possessed a broader pH and temperature range when compared with Pa×gGolSII. Interestingly, spatial and temporal expression analyses revealed that Pa×gGolSII transcript levels varied seasonally, while Pa×gGolSI did not, implying temperature-regulated transcriptional control of this gene in addition to the observed thermosensitivity of the respective enzyme. This evidence suggested that Pa×gGolSI may be involved in basic metabolic activities such as storage, while Pa×gGolSII is probably involved in seasonal mobilization of carbohydrates.
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Registered trials
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