ArticlePlant & cell physiology2012
Reference genes for the normalization of gene expression in eucalyptus species.
Article in Plant & cell physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed.
- Identification of the phosphate transporter 1 family genes in the Eucalyptus grandis genome and their expression under different phosphate regimes.BMC genomics · 2026Article
- Validation of reliable reference genes for comparison of gene expression across species in the Anopheles Hyrcanus Group.Scientific reports · 2025Article
- Identification of the Optimal Quantitative RT-PCR Reference Gene for Paper Mulberry (Current issues in molecular biology · 2024Article
- Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis.BMC plant biology · 2024Article
- Reference genes for Eucalyptus spp. under Beauveria bassiana inoculation and subsequently infestation by the galling wasp Leptocybe invasa.Scientific reports · 2024Article
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- Development of reference genes for RT-qPCR analysis of gene expression in Pleurotus pulmonarius for biotechnological applications.Scientific reports · 2023Article
- Selection and verification of reliable internal reference genes in stem development of herbaceous peony (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Article
- Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.).Molecular biology reports · 2022Article
- Identification and Validation of Reliable Reference Genes for Gene Expression Studies inGenes · 2022Article
- Screening of stable internal reference gene of Quinoa under hormone treatment and abiotic stress.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Selection and validation of endogenous reference genes for RT-qPCR normalization in different stresses and tissues of the tiger milk mushroom,Mycoscience · 2021Article
- Article
- Selection and Validation of the Optimal Panel of Reference Genes for RT-qPCR Analysis in the Developing Rat Cartilage.Frontiers in genetics · 2020Article
- Reference gene selection for real-time quantitative PCR normalization in Hemarthria compressa and Hemarthria altissima leaf tissue.Molecular biology reports · 2019Article
- Genome-Wide Characterization, Evolution, and Expression Profiling ofInternational journal of molecular sciences · 2019Article
- Comparison of Reliable Reference Genes Following Different Hormone Treatments by Various Algorithms for qRT-PCR Analysis ofInternational journal of molecular sciences · 2018Article
- Temporal regulation of terpene synthase gene expression inEnvironmental and experimental botany · 2018Article
- Characterization ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
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7 authors.
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Abstract
Gene expression analysis is increasingly important in biological research, with reverse transcription-quantitative PCR (RT-qPCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Considering the increased sensitivity, reproducibility and large dynamic range of this method, the requirements for proper internal reference gene(s) for relative expression normalization have become much more stringent. Given the increasing interest in the functional genomics of Eucalyptus, we sought to identify and experimentally verify suitable reference genes for the normalization of gene expression associated with the flower, leaf and xylem of six species of the genus. We selected 50 genes that exhibited the least variation in microarrays of E. grandis leaves and xylem, and E. globulus xylem. We further performed the experimental analysis using RT-qPCR for six Eucalyptus species and three different organs/tissues. Employing algorithms geNorm and NormFinder, we assessed the gene expression stability of eight candidate new reference genes. Classic housekeeping genes were also included in the analysis. The stability profiles of candidate genes were in very good agreement. PCR results proved that the expression of novel Eucons04, Eucons08 and Eucons21 genes was the most stable in all Eucalyptus organs/tissues and species studied. We showed that the combination of these genes as references when measuring the expression of a test gene results in more reliable patterns of expression than traditional housekeeping genes. Hence, novel Eucons04, Eucons08 and Eucons21 genes are the best suitable references for the normalization of expression studies in the Eucalyptus genus.
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