Evidence map›Paper›PMID 22197204›Full record

ArticleUrology2012

Label retaining and stem cell marker expression in the developing rat urinary bladder.

Haiyang Zhang, Guiting Lin, Xuefeng Qiu, Hongxiu Ning, Lia Banie, Tom F Lue, Ching-Shwun Lin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Urology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 37 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Sleeping beauty: awakening urothelium from its slumber.American journal of physiology. Renal physiology · 2017
    Review
  5. Article
  6. Urothelial generation and regeneration in development, injury, and cancer.Developmental dynamics : an official publication of the American Association of Anatomists · 2017
    Review
  7. Review
  8. Presence of stem/progenitor cells in the rat penis.Stem cells and development · 2015
    Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Haiyang ZhangKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143-0738, USA.
Guiting Lin
Xuefeng Qiu
Hongxiu Ning
Lia Banie
Tom F Lue
Ching-Shwun Lin
University of California, San Francisco · USNanjing Drum Tower Hospital · CNShandong University · CN

Funding

Urinary Incontinence: Reproductive/Hormonal Risk FactorsP50DK064538 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BROWN, JEANETTE S · 2002 to 2011
$11.5M
Prevention and Treatment of ImpotenceR37DK045370 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LUE, TOM F · 2004 to 2013
$3.5M
Mechanism and Prevention of Female Stress Urinary IncontinenceR01DK069655 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LUE, TOM F · 2005 to 2013
$3.1M
MOLECULAR MECHANISM FEMALE STRESS URINARY INCONTINENCER01DK051374 · NIDDK · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI LUE, TOM F · 1999 to 2002
$913k
TREATMENT AND PREVENTION OF IMPOTENCER01DK045370 · NIDDK · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI LUE, TOM F · 1994 to 2001
$631k
NIDDK NIH HHS DK045370NIDDK NIH HHS DK069655NIDDK NIH HHS DK64538NIDDK NIH HHS P50 DK064538NIDDK NIH HHS R01 DK045370NIDDK NIH HHS R01 DK051374NIDDK NIH HHS R01 DK069655NIDDK NIH HHS R37 DK045370
6 · The paper itself

Abstract

objectiveTo identify potential stem cells in urinary bladder by label-retaining cell (LRC) strategy and immunostaining for putative stem cell markers.

methodsNewborn rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU) and their bladders harvested at 4 different time points afterward. The bladders were processed for EdU staining and immunofluorescence staining for stem cell markers Lgr5, CD34, SSEA-1, and c-kit. EdU-positive cells were counted and colocalization with stem cell markers determined.

resultsAt day 1 post-EdU injection, 1804.0 ± 227.7 bladder cells were labeled in each cross-section. As time increased, fewer bladders remained labeled, dropping to 236.5 ± 53.0 cells per field. In the 1-day bladders, 27.5% ± 4.9% of the epithelial cells were labeled as compared to 12.1% ± 2.8% in the detrusor. The labeling rates in these 2 tissue compartments gradually equalized, reaching at approximately 5.5% in the 8-week samples. Distribution of LRC was random, without preferential labeling of basal cells. Lgr5 and SSEA-1 were detectable in the urothelium, and CD34 and c-kit in the lamina propria and detrusor. Approximately 30%-40% of c-kit-positive cells were EdU positive.

conclusionLabeling of bladder cells by EdU occurred randomly, and label retaining was not associated with expression of Lgr5, CD34, or SSEA-1. The strong association between label retaining and c-kit expression appears to relate to interstitial cells of Cajal, not stem cells.

Indexed as

Age FactorsAnimalsAnimals, NewbornAntigens, CD34BiomarkersDeoxyuridineFluorescent Antibody TechniqueLewis X AntigenMucous MembraneMuscle, SmoothProto-Oncogene Proteins c-kitRatsRats, Sprague-DawleyReceptors, G-Protein-CoupledStem CellsUrinary Bladder5-ethynyl-2'-deoxyuridineAntigens, CD34BiomarkersDeoxyuridineLewis X AntigenProto-Oncogene Proteins c-kitReceptors, G-Protein-Coupled

Identifiers

PMID22197204
PMCPMC3294032
OpenAlexW2017107177

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.