ArticleJournal of inorganic biochemistry2012
Characterization of Zn(II)-responsive ribosomal proteins YkgM and L31 in E. coli.
Article in Journal of inorganic biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
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- One-carbon metabolism and microbial pathogenicity.Molecular oral microbiology · 2024Review
- Chemoproteomics Reveals Disruption of Metal Homeostasis and Metalloproteins by the Antibiotic Holomycin.ACS chemical biology · 2023Article
- The Discovery of Ribosomal Protein bL31 fromInternational journal of molecular sciences · 2023Review
- Ribosome Protein Composition Mediates Translation during theInternational journal of molecular sciences · 2023Article
- Zur and zinc increase expression of E. coli ribosomal protein L31 through RNA-mediated repression of the repressor L31p.Nucleic acids research · 2022Article
- Vibrio cholerae's mysterious Seventh Pandemic island (VSP-II) encodes novel Zur-regulated zinc starvation genes involved in chemotaxis and cell congregation.PLoS genetics · 2021Article
- Grad-seq shines light on unrecognized RNA and protein complexes in the model bacterium Escherichia coli.Nucleic acids research · 2020Article
- Phenotypic effects of paralogous ribosomal proteins bL31A and bL31B in E. coli.Scientific reports · 2020Article
- One-carbon metabolism, folate, zinc and translation.Microbial biotechnology · 2020Article
- Zinc, an unexpected integrator of metabolism?Microbial biotechnology · 2020Article
- Autogenous regulation in vivo of theRNA (New York, N.Y.) · 2020Article
- The Ribosome as a Switchboard for Bacterial Stress Response.Frontiers in microbiology · 2020Review
- Transcriptome dynamics of theeLife · 2019Article
- Does functional specialization of ribosomes really exist?RNA (New York, N.Y.) · 2019Review
- Endopeptidase Regulation as a Novel Function of the Zur-Dependent Zinc Starvation Response.mBio · 2019Article
- Effects of a Four-Week High-Dosage Zinc Oxide Supplemented Diet on CommensalFrontiers in microbiology · 2019Article
- Native Proteomics in Discovery Mode Using Size-Exclusion Chromatography-Capillary Zone Electrophoresis-Tandem Mass Spectrometry.Analytical chemistry · 2018Article
- Zinc depletion induces ribosome hibernation in mycobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
RT-PCR and DNA microarrays were used to probe for Zn(II)-responsive genes in E. coli cells that were made Zn(II) deficient. Microarray data revealed 114 genes were significantly up-regulated and 146 genes were significantly down-regulated in Zn(II) deficient conditions. The three most up-regulated genes were (1) znuA, which encodes for a periplasmic protein known to be involved with Zn(II) import, (2) yodA, which encodes for a periplasmic protein with unknown function, and (3) ykgM, which encodes for a ribosomal protein that is thought to be a paralog of ribosomal protein L31. YodA was over-expressed and purified as a maltose binding protein (MBP) fusion protein and shown to tightly bind 4 equivalents of Zn(II). Metal analyses showed that MBP-YkgM does not bind Zn(II). On the other hand, MBP-L31 tightly binds 1 equivalent of Zn(II). EXAFS studies on MBP-L31 suggest a ligand field of 1 histidine, 1 cysteine, and 2 additional N/O scatterers. Site-directed mutagenesis studies suggest that Cys16 coordinates Zn(II) in MBP-L31 and that the other three cysteines do not bind metal. These results are discussed in light of Zn(II) starvation model that has been postulated for B. subtilis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.