ArticlePloS one2011
Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Laboratory strategies for the identification of Burkholderia species: From classical phenotyping to advanced genomic and proteomic approaches.Antonie van Leeuwenhoek · 2026Review
- Emergence of Human and Animal Melioidosis in Southern Africa, 2018-2021.Tropical medicine and infectious disease · 2026Article
- A novel ready-to-use loop-mediated isothermal amplification (LAMP) method for detection of Burkholderia mallei and B. pseudomallei.BMC microbiology · 2025Article
- High-resolution phylogenetic and population genetic analysis of microbial communities with RoC-ITS.ISME communications · 2022Article
- In silico analyses of penicillin binding proteins in Burkholderia pseudomallei uncovers SNPs with utility for phylogeography, species differentiation, and sequence typing.PLoS neglected tropical diseases · 2022Article
- 16S rDNA and ITS Sequence Diversity of Burkholderia mallei Isolated from Glanders-Affected Horses and Mules in India (2013-2019).Current microbiology · 2021Article
- Human Melioidosis.Clinical microbiology reviews · 2020Review
- Burkholderia pseudomallei, the causative agent of melioidosis, is rare but ecologically established and widely dispersed in the environment in Puerto Rico.PLoS neglected tropical diseases · 2019Article
- Pangenomic Definition of Prokaryotic Species and the Phylogenetic Structure ofFrontiers in microbiology · 2018Article
- Phylogeography of Burkholderia pseudomallei Isolates, Western Hemisphere.Emerging infectious diseases · 2017Article
- Comparing in vitro and in vivo virulence phenotypes of Burkholderia pseudomallei type G strains.PloS one · 2017Article
- Unprecedented Melioidosis Cases in Northern Australia Caused by an Asian Burkholderia pseudomallei Strain Identified by Using Large-Scale Comparative Genomics.Applied and environmental microbiology · 2016Article
- A Review of Melioidosis Cases in the Americas.The American journal of tropical medicine and hygiene · 2015Review
- Isolation of the highly pathogenic and zoonotic agent Burkholderia pseudomallei from a pet green Iguana in Prague, Czech Republic.BMC veterinary research · 2014Article
- Fatal Burkholderia pseudomallei infection initially reported as a Bacillus species, Ohio, 2013.The American journal of tropical medicine and hygiene · 2014Article
- Distribution of Burkholderia pseudomallei in northern Australia, a land of diversity.Applied and environmental microbiology · 2014Article
- Kind discrimination and competitive exclusion mediated by contact-dependent growth inhibition systems shape biofilm community structure.PLoS pathogens · 2014Article
- Burkholderia pseudomallei type G in Western Hemisphere.Emerging infectious diseases · 2014Article
- Recombination drives evolution of the Clostridium difficile 16S-23S rRNA intergenic spacer region.PloS one · 2014Article
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14 authors.
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Abstract
Length polymorphisms within the 16S-23S ribosomal DNA internal transcribed spacer (ITS) have been described as stable genetic markers for studying bacterial phylogenetics. In this study, we used these genetic markers to investigate phylogenetic relationships in Burkholderia pseudomallei and its near-relative species. B. pseudomallei is known as one of the most genetically recombined bacterial species. In silico analysis of multiple B. pseudomallei genomes revealed approximately four homologous rRNA operons and ITS length polymorphisms therein. We characterized ITS distribution using PCR and analyzed via a high-throughput capillary electrophoresis in 1,191 B. pseudomallei strains. Three major ITS types were identified, two of which were commonly found in most B. pseudomallei strains from the endemic areas, whereas the third one was significantly correlated with worldwide sporadic strains. Interestingly, mixtures of the two common ITS types were observed within the same strains, and at a greater incidence in Thailand than Australia suggesting that genetic recombination causes the ITS variation within species, with greater recombination frequency in Thailand. In addition, the B. mallei ITS type was common to B. pseudomallei, providing further support that B. mallei is a clone of B. pseudomallei. Other B. pseudomallei near-neighbors possessed unique and monomorphic ITS types. Our data shed light on evolutionary patterns of B. pseudomallei and its near relative species.
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