ArticlePloS one2011
Conditional gene expression in Mycobacterium abscessus.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
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- Construction of a Subcutaneous Fat Transplantation Model Infected with Mycobacterium.Current medicinal chemistry · 2026Article
- Status quo of tet regulation in bacteria.Microbial biotechnology · 2022Review
- Repressible Promoter System to Study Essential Genes in Mycobacteria.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Genome-Wide Essentiality Analysis ofmBio · 2021Article
- Lsr2 Is an Important Determinant of Intracellular Growth and Virulence inFrontiers in microbiology · 2019Article
- Screening of Preselected Libraries Targeting Mycobacterium abscessus for Drug Discovery.Antimicrobial agents and chemotherapy · 2018Article
- Glycopeptidolipids, a Double-Edged Sword of theFrontiers in microbiology · 2018Review
- Molecular Mechanisms of Intrinsic Streptomycin Resistance in Mycobacterium abscessus.Antimicrobial agents and chemotherapy · 2018Article
- Promoter mutagenesis for fine-tuning expression of essential genes in Mycobacterium tuberculosis.Microbial biotechnology · 2018Article
- A Simple and Rapid Gene Disruption Strategy inFrontiers in cellular and infection microbiology · 2018Article
- Ser/Thr Phosphorylation Regulates the Fatty Acyl-AMP Ligase Activity of FadD32, an Essential Enzyme in Mycolic Acid Biosynthesis.The Journal of biological chemistry · 2016Article
- In vivo assessment of drug efficacy against Mycobacterium abscessus using the embryonic zebrafish test system.Antimicrobial agents and chemotherapy · 2014Article
- Mycobacterium abscessus cording prevents phagocytosis and promotes abscess formation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Regulated Expression Systems for Mycobacteria and Their Applications.Microbiology spectrum · 2014Article
- Gene replacement in Mycobacterium chelonae: application to the construction of porin knock-out mutants.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterium abscessus is an emerging human pathogen responsible for lung infections, skin and soft-tissue infections and disseminated infections in immunocompromised patients. It may exist either as a smooth (S) or rough (R) morphotype, the latter being associated with increased pathogenicity in various models. Genetic tools for homologous recombination and conditional gene expression are desperately needed to allow the study of M. abscessus virulence. However, descriptions of knock-out (KO) mutants in M. abscessus are rare, with only one KO mutant from an S strain described so far. Moreover, of the three major tools developed for homologous recombination in mycobacteria, only the one based on expression of phage recombinases is working. Several conditional gene expression tools have recently been engineered for Mycobacterium tuberculosis and Mycobacterium smegmatis, but none have been tested yet in M. abscessus. Based on previous experience with genetic tools allowing homologous recombination and their failure in M. abscessus, we evaluated the potential interest of a conditional gene expression approach using a system derived from the two repressors system, TetR/PipOFF. After several steps necessary to adapt TetR/PipOFF for M. abscessus, we have shown the efficiency of this system for conditional expression of an essential mycobacterial gene, fadD32. Inhibition of fadD32 was demonstrated for both the S and R isotypes, with marginally better efficiency for the R isotype. Conditional gene expression using the dedicated TetR/PipOFF system vectors developed here is effective in S and R M. abscessus, and may constitute an interesting approach for future genetic studies in this pathogen.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.