ArticlePloS one2011
Spatial regulation of exocytic site and vesicle mobilization by the actin cytoskeleton.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Activation and expansion of presynaptic signaling foci drives presynaptic homeostatic plasticity.Neuron · 2022Article
- The actin binding protein scinderin acts in PC12 cells to tether dense-core vesicles prior to secretion.Molecular and cellular neurosciences · 2017Article
- Podocyte Purinergic P2X4 Channels Are Mechanotransducers That Mediate Cytoskeletal Disorganization.Journal of the American Society of Nephrology : JASN · 2016Article
- Spatiotemporal detection and analysis of exocytosis reveal fusion "hotspots" organized by the cytoskeleton in endocrine cells.Biophysical journal · 2015Article
- Systematic spatial mapping of proteins at exocytic and endocytic structures.Molecular biology of the cell · 2014Article
- Actin controls the vesicular fraction of dopamine released during extended kiss and run exocytosis.ACS chemical biology · 2014Article
- Amperometric measurements at cells support a role for dynamin in the dilation of the fusion pore during exocytosis.Chemphyschem : a European journal of chemical physics and physical chemistry · 2013Article
- Membrane cholesterol removal changes mechanical properties of cells and induces secretion of a specific pool of lysosomes.PloS one · 2013Article
- NK cell lytic granules are highly motile at the immunological synapse and require F-actin for post-degranulation persistence.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Numerous studies indicate a role for the actin cytoskeleton in secretion. Here, we have used evanescent wave and widefield fluorescence microscopy to study the involvement of the actin cytoskeleton in secretion from PC12 cells. Secretion was assayed as loss of ANF-EmGFP in widefield mode. Under control conditions, depolarization induced secretion showed two phases: an initial rapid rate of loss of vesicular cargo (tau = 1.4 s), followed by a slower, sustained drop in fluorescence (tau = 34.1 s). Pretreatment with Latrunculin A changed the kinetics to a single exponential, slightly faster than the fast component of control cells (1.2 s). Evanescent wave microscopy allowed us to examine this at the level of individual events, and revealed equivalent changes in the rates of vesicular arrival at the plasma membrane immediately following and during the sustained phase of release. Co-transfection of mCherry labeled β-actin and ANF-EmGFP demonstrated that sites of exocytosis had an inverse relationship with sites of actin enrichment. Disruption of visualized actin at the membrane resulted in the loss of specificity of exocytic site.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.