ArticlePloS one2011
Probing the production of amidated peptides following genetic and dietary copper manipulations.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Peptidylglycine alpha-amidating monooxygenase is important in mice for beta-cell cilia formation and insulin secretion but promotes diabetes risk through beta-cell independent mechanisms.Molecular metabolism · 2025Article
- Combined copper and zinc deficiency is associated with reduced SARS-CoV-2 immunization response to BNT162b2 vaccination.Heliyon · 2023Article
- Translational and Posttranslational Dynamics in a Model Peptidergic System.Molecular & cellular proteomics : MCP · 2023Article
- Germline loss-of-functionmedRxiv : the preprint server for health sciences · 2023Article
- Article
- Germline loss-of-functionFrontiers in endocrinology · 2023Article
- Short-Term Administration of Common Anesthetics Does Not Dramatically Change the Endogenous Peptide Profile in the Rat Pituitary.ACS chemical neuroscience · 2022Article
- Peptidylglycine α-amidating monooxygenase as a therapeutic target or biomarker for human diseases.British journal of pharmacology · 2022Review
- Novel insights into peptide amidation and amidating activity in the human circulation.Scientific reports · 2021Article
- Effects of maternal immune activation in porcine transcript isoforms of neuropeptide and receptor genes.Journal of integrative neuroscience · 2021Article
- Optimizing production of Fc-amidated peptides by Chinese hamster ovary cells.BMC biotechnology · 2015Article
- Pam heterozygous mice reveal essential role for Cu in amygdalar behavioral and synaptic function.Annals of the New York Academy of Sciences · 2014Article
- In vivo and in vitro analyses of amygdalar function reveal a role for copper.Journal of neurophysiology · 2014Article
- Identification of low-frequency and rare sequence variants associated with elevated or reduced risk of type 2 diabetes.Nature genetics · 2014Article
- Accurate assignment of significance to neuropeptide identifications using Monte Carlo k-permuted decoy databases.PloS one · 2014Article
- Characterization of the peptidylglycine α-amidating monooxygenase (PAM) from the venom ducts of neogastropods, Conus bullatus and Conus geographus.Toxicon : official journal of the International Society on Toxinology · 2013Article
- Quantitation of endogenous peptides using mass spectrometry based methods.Current opinion in chemical biology · 2013Review
- Article
- Signaling from the secretory granule to the nucleus.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Amidated neuropeptides play essential roles throughout the nervous and endocrine systems. Mice lacking peptidylglycine α-amidating monooxygenase (PAM), the only enzyme capable of producing amidated peptides, are not viable. In the amidation reaction, the reactant (glycine-extended peptide) is converted into a reaction intermediate (hydroxyglycine-extended peptide) by the copper-dependent peptidylglycine-α-hydroxylating monooxygenase (PHM) domain of PAM. The hydroxyglycine-extended peptide is then converted into amidated product by the peptidyl-α-hydroxyglycine α-amidating lyase (PAL) domain of PAM. PHM and PAL are stitched together in vertebrates, but separated in some invertebrates such as Drosophila and Hydra. In addition to its luminal catalytic domains, PAM includes a cytosolic domain that can enter the nucleus following release from the membrane by γ-secretase. In this work, several glycine- and hydroxyglycine-extended peptides as well as amidated peptides were qualitatively and quantitatively assessed from pituitaries of wild-type mice and mice with a single copy of the Pam gene (PAM(+/-)) via liquid chromatography-mass spectrometry-based methods. We provide the first evidence for the presence of a peptidyl-α-hydroxyglycine in vivo, indicating that the reaction intermediate becomes free and is not handed directly from PHM to PAL in vertebrates. Wild-type mice fed a copper deficient diet and PAM(+/-) mice exhibit similar behavioral deficits. While glycine-extended reaction intermediates accumulated in the PAM(+/-) mice and reflected dietary copper availability, amidated products were far more prevalent under the conditions examined, suggesting that the behavioral deficits observed do not simply reflect a lack of amidated peptides.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.