ArticlePloS one2011
Antibiotic susceptibility testing of grown blood cultures by combining culture and real-time polymerase chain reaction is rapid and effective.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Impact of same-day antibiotic susceptibility testing on time to appropriate antibiotic treatment of patients with bacteraemia: a randomised controlled trial.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Trial
- Emerging Diabetic Foot Ulcer Microbiome Analysis Using Cutting Edge Technologies.Journal of diabetes science and technology · 2022Article
- Modern Blood Culture: Management Decisions and Method Options.Clinics in laboratory medicine · 2020Review
- Analysis Of Blood Culture Results Of Burn Septicaemia Patients Over A Period Of Nine Years In The Baghdad Burn Medical Centre.Annals of burns and fire disasters · 2020Article
- Nanoarray Digital Polymerase Chain Reaction with High-Resolution Melt for Enabling Broad Bacteria Identification and Pheno-Molecular Antimicrobial Susceptibility Test.Analytical chemistry · 2019Article
- Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction.PloS one · 2018Article
- Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing.SLAS technology · 2017Article
- Development of a Rapid Reverse Blot Hybridization Assay for Detection of Clinically Relevant Antibiotic Resistance Genes in Blood Cultures Testing Positive for Gram-Negative Bacteria.Frontiers in microbiology · 2017Article
- Feasibility study demonstrating that enzymatic template generation and amplification can be employed as a novel method for molecular antimicrobial susceptibility testing.BMC microbiology · 2013Article
- Real-time optical antimicrobial susceptibility testing.Journal of clinical microbiology · 2013Article
- Molecular detection of CF lung pathogens: current status and future potential.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2013Review
- Rapid clinical bacteriology and its future impact.Annals of laboratory medicine · 2013Review
- One-day workflow scheme for bacterial pathogen detection and antimicrobial resistance testing from blood cultures.Journal of visualized experiments : JoVE · 2012Article
- A 'culture' shift: Application of molecular techniques for diagnosing polymicrobial infections.Biotechnology advancesReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEarly administration of appropriate antibiotic therapy in bacteraemia patients dramatically reduces mortality. A new method for RApid Molecular Antibiotic Susceptibility Testing (RAMAST) that can be applied directly to positive blood cultures was developed and evaluated. METHODOLOGY/PRINCIPAL
findingsGrowth curves and antibiotic susceptibility of blood culture isolates (Staphylococcus aureus, enterococci and (facultative) aerobic gram-negative rods) were determined by incubating diluted blood cultures with and without antibiotics, followed by a quantitative universal 16S PCR to detect the presence or absence of growth. Testing 114 positive blood cultures, RAMAST showed an agreement with microbroth dilution of 96.7% for gram-negative rods, with a minor error (false-susceptibility with a intermediate resistant strain) rate of 1.9%, a major error (false resistance) rate of 0.8% and a very major error (false susceptibility) rate of 0.6%. Agreement for S. aureus was 97.9%, with a very major error rate of 2.1%. Enterococcus species showed 95.0% agreement, with a major error rate of 5.0%. These agreements are comparable with those of the Phoenix system. Starting from a positive blood culture, the test was completed within 9 hours. CONCLUSIONS/SIGNIFICANCE: This new rapid method for antibiotic susceptibility testing can potentially provide accurate results for most relevant bacteria commonly isolated from positive blood cultures in less time than routine methods.
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