ArticlePloS one2011
Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Vertical transmission of chronic wasting disease in free-ranging white-tailed deer populations.Scientific reports · 2025Article
- Detection of prions in oocytes and ovaries of ewes naturally infected with classical scrapie.Veterinary research · 2025Article
- Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer.Viruses · 2021Article
- Evaluation of Antemortem Diagnostic Techniques in Goats Naturally Infected With Scrapie.Frontiers in veterinary science · 2020Article
- How do PrPPathogens (Basel, Switzerland) · 2017Review
- Infectious Prions in the Pregnancy Microenvironment of Chronic Wasting Disease-Infected Reeves' Muntjac Deer.Journal of virology · 2017Article
- Types and Strains: Their Essential Role in Understanding Protein Aggregation in Neurodegenerative Diseases.Frontiers in aging neuroscience · 2017Review
- Prenatal transmission of scrapie in sheep and goats: A case study for veterinary public health.Open veterinary journal · 2016Review
- Dynamics of the natural transmission of bovine spongiform encephalopathy within an intensively managed sheep flock.Veterinary research · 2015Review
- Evidence of in utero transmission of classical scrapie in sheep.Journal of virology · 2014Article
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- Evidence in sheep for pre-natal transmission of scrapie to lambs from infected mothers.PloS one · 2013Article
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and has rarely been studied. Recently, the use of the protein misfolding cyclic amplification technique (PMCA) has allowed prion detection in various tissues and excretions in which PrPres levels have been undetectable by traditional assays. The main goal of this study was to detect PrPres in fetal tissues and the amniotic fluid from natural scrapie infected ewes using the PMCA technique. Six fetuses from three infected pregnant ewes in an advanced clinical stage of the disease were included in the study. From each fetus, amniotic fluid, brain, spleen, ileo-cecal valve and retropharyngeal lymph node samples were collected and analyzed using Western blotting and PMCA. Although all samples were negative using Western blotting, PrPres was detected after in vitro amplification. Our results represent the first time the biochemical detection of prions in fetal tissues, suggesting that the in utero transmission of scrapie in natural infected sheep might be possible.
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