ArticleThe Journal of biological chemistry2012
Human mitochondrial DNA polymerase γ exhibits potential for bypass and mutagenesis at UV-induced cyclobutane thymine dimers.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Translesion DNA synthesis on pyrimidine dimers by plant organellar DNA polymerases is metal-dependent.Nucleic acids research · 2026Article
- Mitochondrial dysfunction and oxidative stress contribute to cross-generational toxicity of benzo(a)pyrene in Danio rerio.Aquatic toxicology (Amsterdam, Netherlands) · 2023Article
- Rolling Circle Replication and Bypass of Damaged Nucleotides.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Human Mitochondrial DNA Polymerase Metal Dependent UV Lesion Bypassing Ability.Frontiers in molecular biosciences · 2022Article
- Polymerase γ efficiently replicates through many natural template barriers but stalls at the HSP1 quadruplex.The Journal of biological chemistry · 2020Article
- Mitophagy and DNA damage signaling in human aging.Mechanisms of ageing and development · 2020Review
- Plant organellar DNA polymerases are replicative and translesion DNA synthesis polymerases.Nucleic acids research · 2017Article
- DNA damage related crosstalk between the nucleus and mitochondria.Free radical biology & medicine · 2017Review
- Mitochondrial DNA replication: a PrimPol perspective.Biochemical Society transactions · 2017Review
- DNA polymerase θ specializes in incorporating synthetic expanded-size (xDNA) nucleotides.Nucleic acids research · 2016Article
- Effects of reduced mitochondrial DNA content on secondary mitochondrial toxicant exposure in Caenorhabditis elegans.Mitochondrion · 2016Article
- Mitochondrial DNA damage induced autophagy, cell death, and disease.Frontiers in bioscience (Landmark edition) · 2016Review
- Analysis of Translesion DNA Synthesis by the Mitochondrial DNA Polymerase γ.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Mitochondrial DNA maintenance: an appraisal.Molecular and cellular biochemistry · 2015Review
- The role of TDP1 and APTX in mitochondrial DNA repair.Biochimie · 2014Review
- DNA damage response in peripheral nervous system: coping with cancer therapy-induced DNA lesions.DNA repair · 2013Review
- Mitochondria as a target of environmental toxicants.Toxicological sciences : an official journal of the Society of Toxicology · 2013Review
- Translesion synthesis past acrolein-derived DNA adducts by human mitochondrial DNA polymerase γ.The Journal of biological chemistry · 2013Article
- Effects of early life exposure to ultraviolet C radiation on mitochondrial DNA content, transcription, ATP production, and oxygen consumption in developing Caenorhabditis elegans.BMC pharmacology & toxicology · 2013Article
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4 authors.
Funding
Abstract
Cyclobutane thymine dimers (T-T) comprise the majority of DNA damage caused by short wavelength ultraviolet radiation. These lesions generally block replicative DNA polymerases and are repaired by nucleotide excision repair or bypassed by translesion polymerases in the nucleus. Mitochondria lack nucleotide excision repair, and therefore, it is important to understand how the sole mitochondrial DNA polymerase, pol γ, interacts with irreparable lesions such as T-T. We performed in vitro DNA polymerization assays to measure the kinetics of incorporation opposite the lesion and bypass of the lesion by pol γ with a dimer-containing template. Exonuclease-deficient pol γ bypassed thymine dimers with low relative efficiency; bypass was attenuated but still detectable when using exonuclease-proficient pol γ. When bypass did occur, pol γ misincorporated a guanine residue opposite the 3'-thymine of the dimer only 4-fold less efficiently than it incorporated an adenine. Surprisingly, the pol γ exonuclease-proficient enzyme excised the incorrectly incorporated guanine at similar rates irrespective of the nature of the thymines in the template. In the presence of all four dNTPs, pol γ extended the primer after incorporation of two adenines opposite the lesion with relatively higher efficiency compared with extension past either an adenine or a guanine incorporated opposite the 3'-thymine of the T-T. Our results suggest that T-T usually stalls mitochondrial DNA replication but also suggest a mechanism for the introduction of point mutations and deletions in the mitochondrial genomes of chronically UV-exposed cells.
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