ArticleJournal of bacteriology2012
Ribulokinase and transcriptional regulation of arabinose metabolism in Clostridium acetobutylicum.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
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- Genetic sensor-regulators functional in Clostridia.Journal of industrial microbiology & biotechnology · 2020Article
- Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia.Biotechnology for biofuels · 2020Review
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- Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysis.Biotechnology for biofuels · 2019Review
- The Zn2Cys6-type transcription factor LeuB cross-links regulation of leucine biosynthesis and iron acquisition in Aspergillus fumigatus.PLoS genetics · 2018Article
- Comparative Genomics Reveals the Regulatory Complexity of Bifidobacterial Arabinose and Arabino-Oligosaccharide Utilization.Frontiers in microbiology · 2018Article
- Genomic Reconstruction of Carbohydrate Utilization Capacities in Microbial-Mat Derived Consortia.Frontiers in microbiology · 2017Article
- Metabolite labelling reveals hierarchies in Clostridium acetobutylicum that selectively channel carbons from sugar mixtures towards biofuel precursors.Microbial biotechnology · 2017Article
- Sugar uptake by the solventogenic clostridia.World journal of microbiology & biotechnology · 2016Article
- A novel transcriptional regulator of L-arabinose utilization in human gut bacteria.Nucleic acids research · 2015Article
- A novel arabinose-inducible genetic operation system developed for Clostridium cellulolyticum.Biotechnology for biofuels · 2015Article
- GntR Family of Bacterial Transcription Factors and Their DNA Binding Motifs: Structure, Positioning and Co-Evolution.PloS one · 2015Article
- Arabidopsis miR171-targeted scarecrow-like proteins bind to GT cis-elements and mediate gibberellin-regulated chlorophyll biosynthesis under light conditions.PLoS genetics · 2014Article
- Transcriptional regulation of the carbohydrate utilization network in Thermotoga maritima.Frontiers in microbiology · 2013Article
- Functional diversification of ROK-family transcriptional regulators of sugar catabolism in the Thermotogae phylum.Nucleic acids research · 2013Article
- Comparative genomics and functional analysis of rhamnose catabolic pathways and regulons in bacteria.Frontiers in microbiology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcription factor AraR controls utilization of L-arabinose in Bacillus subtilis. In this study, we combined a comparative genomic reconstruction of AraR regulons in nine Clostridium species with detailed experimental characterization of AraR-mediated regulation in Clostridium acetobutylicum. Based on the reconstructed AraR regulons, a novel ribulokinase, AraK, present in all analyzed Clostridium species was identified, which was a nonorthologous replacement of previously characterized ribulokinases. The predicted function of the araK gene was confirmed by inactivation of the araK gene in C. acetobutylicum and biochemical assays using purified recombinant AraK. In addition to the genes involved in arabinose utilization and arabinoside degradation, extension of the AraR regulon to the pentose phosphate pathway genes in several Clostridium species was revealed. The predicted AraR-binding sites in the C. acetobutylicum genome and the negative effect of L-arabinose on DNA-regulator complex formation were verified by in vitro binding assays. The predicted AraR-controlled genes in C. acetobutylicum were experimentally validated by testing gene expression patterns in both wild-type and araR-inactivated mutant strains during growth in the absence or presence of L-arabinose.
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