ArticleJournal of bacteriology2012
Helicobacter pylori relies primarily on the purine salvage pathway for purine nucleotide biosynthesis.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 93 citations in OpenAlex.
- De novo or Salvage? Nucleotide Availability as a Driver of Bacterial Adaptation and Virulence.MicrobiologyOpen · 2026Review
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- Multistep loss of catalytic and ligand binding abilities of hexameric purine nucleoside phosphorylase.Scientific reports · 2026Article
- Metabolic interactions between bacterial co-isolates from catheter-associated urinary tract infections.Scientific reports · 2026Article
- Challenges and Prospects for Eradication ofPathogens (Basel, Switzerland) · 2025Review
- A thiouracil desulfurase protects Clostridioides difficile RNA from 4-thiouracil incorporation, providing a competitive advantage in the gut.Cell host & microbe · 2025Article
- Vulnerable Nucleotide Pools and Genomic Instability in Yeast Strains with Deletion of theInternational journal of molecular sciences · 2025Article
- Crystal structure of dihydroorotate dehydrogenase from Helicobacter pylori with bound flavin mononucleotide.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism.BMC oral health · 2025Article
- Vitamin B6 inhibits activity ofJournal of enzyme inhibition and medicinal chemistry · 2024Article
- Location Is Everything: Influence of His-Tag Fusion Site on Properties of Adenylosuccinate Synthetase fromInternational journal of molecular sciences · 2024Article
- Development of Prolinol Containing Inhibitors of Hypoxanthine-Guanine-Xanthine Phosphoribosyltransferase: Rational Structure-Based Drug Design.Journal of medicinal chemistry · 2024Article
- Multitasking functions of bacterial extracellular DNA in biofilms.Journal of bacteriology · 2024Review
- The timing of bacterial mesophyll infection shapes the leaf chemical landscape.Microbiology spectrum · 2024Article
- Preliminary study on the potential impact of probiotic combination therapy onFrontiers in microbiology · 2024Article
- Interactions of 2,6-substituted purines with purine nucleoside phosphorylase fromJournal of enzyme inhibition and medicinal chemistry · 2022Article
- De novo purine nucleotide biosynthesis mediated by MoAde4 is required for conidiation, host colonization and pathogenicity in Magnaporthe oryzae.Applied microbiology and biotechnology · 2022Article
- A comprehensive method for determining cellular uptake of purine nucleoside phosphorylase and adenylosuccinate synthetase inhibitors by H. pylori.Applied microbiology and biotechnology · 2021Article
- Similar solutions to a common challenge: regulation of genes encoding Ralstonia solanacearum xanthine dehydrogenase.FEMS microbiology letters · 2021Article
- Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm.Scientific reports · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Helicobacter pylori is a chronic colonizer of the gastric epithelium and plays a major role in the development of gastritis, peptic ulcer disease, and gastric cancer. In its coevolution with humans, the streamlining of the H. pylori genome has resulted in a significant reduction in metabolic pathways, one being purine nucleotide biosynthesis. Bioinformatic analysis has revealed that H. pylori lacks the enzymatic machinery for de novo production of IMP, the first purine nucleotide formed during GTP and ATP biosynthesis. This suggests that H. pylori must rely heavily on salvage of purines from the environment. In this study, we deleted several genes putatively involved in purine salvage and processing. The growth and survival of these mutants were analyzed in both nutrient-rich and minimal media, and the results confirmed the presence of a robust purine salvage pathway in H. pylori. Of the two phosphoribosyltransferase genes found in the H. pylori genome, only gpt appears to be essential, and an Δapt mutant strain was still capable of growth on adenine, suggesting that adenine processing via Apt is not essential. Deletion of the putative nucleoside phosphorylase gene deoD resulted in an inability of H. pylori to grow on purine nucleosides or the purine base adenine. Our results suggest a purine requirement for growth of H. pylori in standard media, indicating that H. pylori possesses the ability to utilize purines and nucleosides from the environment in the absence of a de novo purine nucleotide biosynthesis pathway.
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