ArticleJournal of bacteriology2012
Sinorhizobium meliloti CheA complexed with CheS exhibits enhanced binding to CheY1, resulting in accelerated CheY1 dephosphorylation.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Review
- CheD is a Key Protein Involved in Regulating the Chemotaxis and Pathogenic Life Cycle of Agrobacterium fabrum.Current microbiology · 2025Article
- A cyanobacterial chemotaxis-like system controls phototactic orientation via phosphorylation of two antagonistic response regulators.microLife · 2024Article
- Azorhizobium caulinodans Chemotaxis Is Controlled by an Unusual Phosphorelay Network.Journal of bacteriology · 2022Article
- The Only Chemoreceptor Encoded byMicroorganisms · 2021Article
- Diversity of bacterial chemosensory systems.Current opinion in microbiology · 2021Review
- The Divergent Key Residues of TwoMicroorganisms · 2021Article
- Rhizobial Chemotaxis and Motility Systems at Work in the Soil.Frontiers in plant science · 2021Review
- CheY1 and CheY2 of Azorhizobium caulinodans ORS571 Regulate Chemotaxis and Competitive Colonization with the Host Plant.Applied and environmental microbiology · 2020Article
- Cellular Stoichiometry of Chemotaxis Proteins inJournal of bacteriology · 2020Article
- Chemotactic Host-Finding Strategies of Plant Endoparasites and Endophytes.Frontiers in plant science · 2020Review
- An Updated Perspective onFrontiers in microbiology · 2020Article
- Cellular Stoichiometry of Methyl-Accepting Chemotaxis Proteins in Sinorhizobium meliloti.Journal of bacteriology · 2018Article
- AApplied and environmental microbiology · 2018Article
- Chemotaxis signaling systems in model beneficial plant-bacteria associations.Plant molecular biology · 2016Review
- Phosphate sink containing two-component signaling systems as tunable threshold devices.PLoS computational biology · 2014Article
- Sinorhizobium meliloti chemoreceptor McpU mediates chemotaxis toward host plant exudates through direct proline sensing.Applied and environmental microbiology · 2014Article
- Phosphorelays provide tunable signal processing capabilities for the cell.PLoS computational biology · 2013Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retrophosphorylation of the histidine kinase CheA in the chemosensory transduction chain is a widespread mechanism for efficient dephosphorylation of the activated response regulator. First discovered in Sinorhizobium meliloti, the main response regulator CheY2-P shuttles its phosphoryl group back to CheA, while a second response regulator, CheY1, serves as a sink for surplus phosphoryl groups from CheA-P. We have identified a new component in this phospho-relay system, a small 97-amino-acid protein named CheS. CheS has no counterpart in enteric bacteria but revealed distinct similarities to proteins of unknown function in other members of the α subgroup of proteobacteria. Deletion of cheS causes a phenotype similar to that of a cheY1 deletion strain. Fluorescence microscopy revealed that CheS is part of the polar chemosensory cluster and that its cellular localization is dependent on the presence of CheA. In vitro binding, as well as coexpression and copurification studies, gave evidence of CheA/CheS complex formation. Using limited proteolysis coupled with mass spectrometric analyses, we defined CheA(163-256) to be the CheS binding domain, which overlaps with the N-terminal part of the CheY2 binding domain (CheA(174-316)). Phosphotransfer experiments using isolated CheA-P showed that dephosphorylation of CheY1-P but not CheY2-P is increased in the presence of CheS. As determined by surface plasmon resonance spectroscopy, CheY1 binds ∼100-fold more strongly to CheA/CheS than to CheA. We propose that CheS facilitates signal termination by enhancing the interaction of CheY1 and CheA, thereby promoting CheY1-P dephosphorylation, which results in a more efficient drainage of the phosphate sink.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.