ArticleJournal of bacteriology2012
Interaction of the histone-like nucleoid structuring protein and the general stress response regulator RpoS at Vibrio cholerae promoters that regulate motility and hemagglutinin/protease expression.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Nucleoid-associated proteins shape the global protein occupancy and transcriptional landscape of a clinical isolate ofmSphere · 2024Article
- Review
- Serotype conversion geneFrontiers in microbiology · 2023Article
- Experimental evolution ofFrontiers in microbiology · 2022Article
- Transcriptomic and phenotype analysis revealed the role ofFrontiers in microbiology · 2022Article
- Bacterial transcription during growth arrest.Transcription · 2021Review
- Motility of Vibrio spp.: regulation and controlling strategies.Applied microbiology and biotechnology · 2020Review
- Regulation of mRNA Stability During Bacterial Stress Responses.Frontiers in microbiology · 2020Review
- Transcriptional regulation of a gonococcal gene encoding a virulence factor (L-lactate permease).PLoS pathogens · 2019Article
- Role of coaggregation in the pathogenicity and prolonged colonisation of Vibrio cholerae.Medical microbiology and immunology · 2019Article
- Article
- The curli regulator CsgD mediates stationary phase counter-silencing of csgBA in Salmonella Typhimurium.Molecular microbiology · 2018Article
- Molecular basis for the differential expression of the global regulator VieA in Vibrio cholerae biotypes directed by H-NS, LeuO and quorum sensing.Molecular microbiology · 2018Article
- Regulatory Hierarchies Controlling Virulence Gene Expression inFrontiers in microbiology · 2018Review
- Article
- Flagellar motility, extracellular proteases and Vibrio cholerae detachment from abiotic and biotic surfaces.Microbial pathogenesis · 2017Article
- H-NS: an overarching regulator of the Vibrio cholerae life cycle.Research in microbiology · 2017Review
- flrA, flrB and flrC regulate adhesion by controlling the expression of critical virulence genes in Vibrio alginolyticus.Emerging microbes & infections · 2016Article
- The Histone-Like Nucleoid Structuring Protein (H-NS) Is a Negative Regulator of the Lateral Flagellar System in the Deep-Sea Bacterium Shewanella piezotolerans WP3.Applied and environmental microbiology · 2016Article
- Vibrio cholerae Biofilms and Cholera Pathogenesis.PLoS neglected tropical diseases · 2016Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The bacterium Vibrio cholerae colonizes the human small intestine and secretes cholera toxin (CT) to cause the rice-watery diarrhea characteristic of this illness. The ability of this pathogen to colonize the small bowel, express CT, and return to the aquatic environment is controlled by a complex network of regulatory proteins. Two global regulators that participate in this process are the histone-like nucleoid structuring protein (H-NS) and the general stress response regulator RpoS. In this study, we address the role of RpoS and H-NS in the coordinate regulation of motility and hemagglutinin (HA)/protease expression. In addition to initiating transcription of hapA encoding HA/protease, RpoS enhanced flrA and rpoN transcription to increase motility. In contrast, H-NS was found to bind to the flrA, rpoN, and hapA promoters and represses their expression. The strength of H-NS repression at the above-mentioned promoters was weaker for hapA, which exhibited the strongest RpoS dependency, suggesting that transcription initiation by RNA polymerase containing σ(S) could be more resistant to H-NS repression. Occupancy of the flrA and hapA promoters by H-NS was demonstrated by chromatin immunoprecipitation (ChIP). We show that the expression of RpoS in the stationary phase significantly diminished H-NS promoter occupancy. Furthermore, RpoS enhanced the transcription of integration host factor (IHF), which positively affected the expression of flrA and rpoN by diminishing the occupancy of H-NS at these promoters. Altogether, we propose a model for RpoS regulation of motility gene expression that involves (i) attenuation of H-NS repression by IHF and (ii) RpoS-dependent transcription initiation resistant to H-NS.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.