ArticleJournal of bacteriology2012
Identification of a new gene required for the biosynthesis of rhodoquinone in Rhodospirillum rubrum.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
- EPR characterization of a metal-binding site in RquA.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Article
- Global distribution of isoprenoid quinones across Bacteria.mSystems · 2026Article
- Article
- UbiN, a novel Rhodobacter capsulatus decarboxylative hydroxylase involved in aerobic ubiquinone biosynthesis.FEBS open bio · 2023Article
- Review
- Microbial rhodoquinone biosynthesis proceeds via an atypical RquA-catalyzed amino transfer from S-adenosyl-L-methionine to ubiquinone.Communications chemistry · 2022Article
- Anaerobic Sulfur Oxidation Underlies Adaptation of a Chemosynthetic Symbiont to Oxic-Anoxic Interfaces.mSystems · 2021Article
- Gregarine single-cell transcriptomics reveals differential mitochondrial remodeling and adaptation in apicomplexans.BMC biology · 2021Article
- Transcriptome Profiling Revealed MultipleFrontiers in microbiology · 2020Article
- Recombinant RquA catalyzes the in vivo conversion of ubiquinone to rhodoquinone in Escherichia coli and Saccharomyces cerevisiae.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2019Article
- The kynurenine pathway is essential for rhodoquinone biosynthesis inThe Journal of biological chemistry · 2019Article
- Rhodoquinone biosynthesis ineLife · 2019Article
- Investigation of candidate genes involved in the rhodoquinone biosynthetic pathway in Rhodospirillum rubrum.PloS one · 2019Article
- Anaerobic respiration coupled with mitochondrial fatty acid synthesis in wax ester fermentation by Euglena gracilis.FEBS letters · 2018Article
- Article
- The Human Gut ColonizerFrontiers in cellular and infection microbiology · 2018Article
- Knockdown of the coenzyme Q synthesis gene Smed-dlp1 affects planarian regeneration and tissue homeostasis.Redox biology · 2015Article
- Diversity and origins of anaerobic metabolism in mitochondria and related organelles.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- The molecular evolution of the Qo motif.Genome biology and evolution · 2014Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Rhodoquinone (RQ) is a required cofactor for anaerobic respiration in Rhodospirillum rubrum, and it is also found in several helminth parasites that utilize a fumarate reductase pathway. RQ is an aminoquinone that is structurally similar to ubiquinone (Q), a polyprenylated benzoquinone used in the aerobic respiratory chain. RQ is not found in humans or other mammals, and therefore, the inhibition of its biosynthesis may provide a novel antiparasitic drug target. To identify a gene specifically required for RQ biosynthesis, we determined the complete genome sequence of a mutant strain of R. rubrum (F11), which cannot grow anaerobically and does not synthesize RQ, and compared it with that of a spontaneous revertant (RF111). RF111 can grow anaerobically and has recovered the ability to synthesize RQ. The two strains differ by a single base pair, which causes a nonsense mutation in the putative methyltransferase gene rquA. To test whether this mutation is important for the F11 phenotype, the wild-type rquA gene was cloned into the pRK404E1 vector and conjugated into F11. Complementation of the anaerobic growth defect in F11 was observed, and liquid chromatography-time of flight mass spectrometry (LC-TOF-MS) analysis of lipid extracts confirmed that plasmid-complemented F11 was able to synthesize RQ. To further validate the requirement of rquA for RQ biosynthesis, we generated a deletion mutant from wild-type R. rubrum by the targeted replacement of rquA with a gentamicin resistance cassette. The ΔrquA mutant exhibited the same phenotype as that of F11. These results are significant because rquA is the first gene to be discovered that is required for RQ biosynthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.