ArticleApplied and environmental microbiology2012
Calcium increases Xylella fastidiosa surface attachment, biofilm formation, and twitching motility.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 164 citations in OpenAlex.
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- XadA-like adhesin XADA2 regulates biofilm formation in X. fastidiosa subsp. fastidiosa putatively by engaging oleic-acid derived oxylipins.Molecular biology reports · 2025Article
- The Role of Heat Shock Protein (Hsp) Chaperones in Environmental Stress Adaptation and Virulence of Plant Pathogenic Bacteria.International journal of molecular sciences · 2025Review
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- Calcium modulation of bacterial wilt disease on potato.Applied and environmental microbiology · 2024Article
- Microfluidic approaches in microbial ecology.Lab on a chip · 2024Review
- Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria.Microorganisms · 2023Review
- Complete functional analysis of type IV pilus components of a reemergent plant pathogen reveals neofunctionalization of paralog genes.PLoS pathogens · 2023Article
- Calcium DeterminesApplied and environmental microbiology · 2022Article
- Thidiazuron: New Trends and Future Perspectives to FightAntibiotics (Basel, Switzerland) · 2022Review
- Effect of calcium hydroxide on morphology and physicochemical properties of Enterococcus faecalis biofilm.Scientific reports · 2022Article
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- The Role of Extracellular DNA in Microbial Attachment to Oxidized Silicon Surfaces in the Presence of CaLangmuir : the ACS journal of surfaces and colloids · 2021Article
- Mycological Investigation of Bottled Water Dispensers in Healthcare Facilities.Pathogens (Basel, Switzerland) · 2021Article
- Flow-assembled chitosan membranes in microfluidics: recent advances and applications.Journal of materials chemistry. B · 2021Review
- Phenotypic and Phylogenetic Characterization of Cu Homeostasis amongPathogens (Basel, Switzerland) · 2021Article
- Functionalized microchannels as xylem-mimicking environment: Quantifying X. fastidiosa cell adhesion.Biophysical journal · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xylella fastidiosa is a plant-pathogenic bacterium that forms biofilms inside xylem vessels, a process thought to be influenced by the chemical composition of xylem sap. In this work, the effect of calcium on the production of X. fastidiosa biofilm and movement was analyzed under in vitro conditions. After a dose-response study with 96-well plates using eight metals, the strongest increase of biofilm formation was observed when medium was supplemented with at least 1.0 mM CaCl(2). The removal of Ca by extracellular (EGTA, 1.5 mM) and intracellular [1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA/AM), 75 μM] chelators reduced biofilm formation without compromising planktonic growth. The concentration of Ca influenced the force of adhesion to the substrate, biofilm thickness, cell-to-cell aggregation, and twitching motility, as shown by assays with microfluidic chambers and other assays. The effect of Ca on attachment was lost when cells were treated with tetracycline, suggesting that Ca has a metabolic or regulatory role in cell adhesion. A double mutant (fimA pilO) lacking type I and type IV pili did not improve biofilm formation or attachment when Ca was added to the medium, while single mutants of type I (fimA) or type IV (pilB) pili formed more biofilm under conditions of higher Ca concentrations. The concentration of Ca in the medium did not significantly influence the levels of exopolysaccharide produced. Our findings indicate that the role of Ca in biofilm formation may be related to the initial surface and cell-to-cell attachment and colonization stages of biofilm establishment, which rely on critical functions by fimbrial structures.
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