ArticleApplied and environmental microbiology2012
Engineering Escherichia coli cells for cellobiose assimilation through a phosphorolytic mechanism.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Advances, challenges, and future directions towards a cellulolytic Escherichia coli.Biotechnology for biofuels and bioproducts · 2026Review
- Glycoside hydrolase-mediated glucomannan catabolism inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Metabolomics andHeliyon · 2024Article
- Engineering of a newMetabolic engineering communications · 2021Article
- Enhanced glycolic acid yield through xylose and cellobiose utilization by metabolically engineered Escherichia coli.Bioprocess and biosystems engineering · 2021Article
- Nutrient dominance governs the assembly of microbial communities in mixed nutrient environments.eLife · 2021Article
- Article
- Genome analyses of uncultured TG2/ZB3 bacteria in 'Margulisbacteria' specifically attached to ectosymbiotic spirochetes of protists in the termite gut.The ISME journal · 2019Article
- Simultaneous consumption of cellobiose and xylose byBiotechnology for biofuels · 2018Article
- Biochemical properties of GH94 cellodextrin phosphorylase THA_1941 from a thermophilic eubacterium Thermosipho africanus TCF52B with cellobiose phosphorylase activity.Scientific reports · 2017Article
- A seven-gene cluster inBiotechnology for biofuels · 2017Article
- Metabolic engineering of Agrobacterium sp. ATCC31749 for curdlan production from cellobiose.Journal of industrial microbiology & biotechnology · 2016Article
- Isobutanol production from cellobionic acid in Escherichia coli.Microbial cell factories · 2015Article
- A paradigm shift in biomass technology from complete to partial cellulose hydrolysis: lessons learned from nature.Bioengineered · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report here the first engineering effort for Escherichia coli biocatalysts to assimilate cellobiose through a phosphorolytic mechanism. Cytoplasmic expression of the Saccharophagus cellobiose phosphorylase was shown to enable E. coli to use cellobiose. Subsequent knockout and complementation studies provided solid evidence that the endogenous LacY was responsible for the transport of cellobiose.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.