ArticleEuropean journal of endocrinology2012
Identification of TPIT and other novel autoantigens in lymphocytic hypophysitis: immunoscreening of a pituitary cDNA library and development of immunoprecipitation assays.
Article in European journal of endocrinology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Unveiling the Etiopathogenic Spectrum of Hypophysitis: A Narrative Review.Journal of personalized medicine · 2023Review
- Autoantibodies involved in primary and secondary adrenal insufficiency following treatment with immune checkpoint inhibitors.Immuno-oncology technology · 2023Review
- Neuroimmunoendocrinology of SARS-CoV 2 Infection.Biomedicines · 2022Review
- Autoantibody reactivity profile of primary autoimmune hypophysitis patients: preliminary results.Endocrine · 2022Article
- Molecular and Genetic Immune Biomarkers of Primary and Immune-Therapy Induced Hypophysitis: From Laboratories to the Clinical Practice.Journal of personalized medicine · 2021Review
- Th17 Cells Contribute to the Pathology of Autoimmune Hypophysitis.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Insights into non-classic and emerging causes of hypopituitarism.Nature reviews. Endocrinology · 2021Review
- Late-Onset Isolated Corticotrope Deficiency in a Woman with Down Syndrome.Case reports in endocrinology · 2021Article
- Impact of Pituitary Autoimmunity and Genetic Disorders on Growth Hormone Deficiency in Children and Adults.International journal of molecular sciences · 2020Review
- The Hypothalamic-Pituitary Axis and Autoantibody Related Disorders.International journal of molecular sciences · 2017Review
- Article
- Revisitation of autoimmune hypophysitis: knowledge and uncertainties on pathophysiological and clinical aspects.Pituitary · 2016Review
- [Hypophysitis : Types and differential diagnosis].Der Pathologe · 2016Review
- Diagnosis and management of tumor-like hypophysitis: A retrospective case series.Oncology letters · 2016Article
- Hypophysitis: Evaluation and Management.Clinical diabetes and endocrinology · 2016Review
- Hypothalamic-Pituitary Autoimmunity and Traumatic Brain Injury.Journal of clinical medicine · 2015Review
- Article
- Endocrine autoimmune diseases and female infertility.Nature reviews. Endocrinology · 2014Review
- Opportunistic Autoimmune Disorders Potentiated by Immune-Checkpoint Inhibitors Anti-CTLA-4 and Anti-PD-1.Frontiers in immunology · 2014Review
- Purification of pituitary autoantigen by column liquid chromatography and chromatofocusing.Central-European journal of immunology · 2014Article
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Authors and funding
8 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLymphocytic hypophysitis is an organ-specific autoimmune disease of the pituitary gland. A specific and sensitive serological test currently does not exist to aid in the diagnosis.
objectiveTo identify target autoantigens in lymphocytic hypophysitis and develop a diagnostic assay for these proteins. DESIGN/
methodsA pituitary cDNA expression library was immunoscreened using sera from four patients with lymphocytic hypophysitis. Relevant cDNA clones from screening, along with previously identified autoantigens pituitary gland-specific factor 1a and 2 (PGSF1a and PGSF2) and neuron-specific enolase (NSE) were tested in an in vitro transcription and translation immunoprecipitation assay. The corticotroph-specific transcription factor, TPIT, was investigated separately as a candidate autoantigen.
resultsSignificantly positive autoantibody reactivity against TPIT was found in 9/86 hypophysitis patients vs 1/90 controls (P = 0.018). The reactivity against TPIT was not specific for lymphocytic hypophysitis with autoantibodies detectable in the sera from patients with other autoimmune endocrine diseases. Autoantibodies were also detected against chromodomain-helicase-DNA binding protein 8, presynaptic cytomatrix protein (piccolo), Ca(2+)-dependent secretion activator, PGSF2 and NSE in serum samples from patients with lymphocytic hypophysitis, but at a frequency that did not differ from healthy controls. Importantly, 8/86 patients with lymphocytic hypophysitis had autoantibodies against any two autoantigens in comparison with 0/90 controls (P = 0.0093).
conclusionsTPIT, a corticotroph-specific transcription factor, was identified as a target autoantigen in 10.5% of patients with lymphocytic hypophysitis. Further autoantigens related to vesicle processing were also identified as potential autoantigens with different immunoreactivity patterns in patients and controls.
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