ArticleJournal of cell science2011
SMRT analysis of MTOC and nuclear positioning reveals the role of EB1 and LIC1 in single-cell polarization.
Article in Journal of cell science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 71 citations in OpenAlex.
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- Autoinhibitory mechanism controls binding of centrosomin motif 1 to γ-tubulin ring complex.The Journal of cell biology · 2023Article
- Actin network architecture can ensure robust centering or sensitive decentering of the centrosome.The EMBO journal · 2022Article
- Interrogating RNA and protein spatial subcellular distribution in smFISH data with DypFISH.Cell reports methods · 2021Article
- A robust unsupervised machine-learning method to quantify the morphological heterogeneity of cells and nuclei.Nature protocols · 2021Article
- A robust unsupervised machine-learning method to quantify the morphological heterogeneity of cells and nuclei.Nature protocols · 2021Article
- Tumor Treating Fields (TTFields) Hinder Cancer Cell Motility through Regulation of Microtubule and Acting Dynamics.Cancers · 2020Article
- Centering and Shifting of Centrosomes in Cells.Cells · 2020Review
- Genome of the webworm Hyphantria cunea unveils genetic adaptations supporting its rapid invasion and spread.BMC genomics · 2020Article
- Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction.The Journal of cell biology · 2018Article
- Paxillin regulates cell polarization and anterograde vesicle trafficking during cell migration.Molecular biology of the cell · 2017Article
- Biophysical and biomolecular determination of cellular age in humans.Nature biomedical engineering · 2017Article
- Centrosome centering and decentering by microtubule network rearrangement.Molecular biology of the cell · 2016Article
- Senescent stromal cells induce cancer cell migration via inhibition of RhoA/ROCK/myosin-based cell contractility.Oncotarget · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
In several migratory cells, the microtubule-organizing center (MTOC) is repositioned between the leading edge and nucleus, creating a polarized morphology. Although our understanding of polarization has progressed as a result of various scratch-wound and cell migration studies, variations in culture conditions required for such assays have prevented a unified understanding of the intricacies of MTOC and nucleus positioning that result in cell polarization. Here, we employ a new SMRT (for sparse, monolayer, round, triangular) analysis that uses a universal coordinate system based on cell centroid to examine the pathways regulating MTOC and nuclear positions in cells plated in a variety of conditions. We find that MTOC and nucleus positioning are crucially and independently affected by cell shape and confluence; MTOC off-centering correlates with the polarization of single cells; acto-myosin contractility and microtubule dynamics are required for single-cell polarization; and end binding protein 1 and light intermediate chain 1, but not Par3 and light intermediate chain 2, are required for single-cell polarization and directional cell motility. Using various cellular geometries and conditions, we implement a systematic and reproducible approach to identify regulators of MTOC and nucleus positioning that depend on extracellular guidance cues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.