ArticleThe Analyst2012
Selective enrichment and sensitive detection of peptide and protein biomarkers in human serum using polymeric reverse micelles and MALDI-MS.
Article in The Analyst, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The trial behind it
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Sequential Nucleophilic "Click" Reactions for Functional Amphiphilic Homopolymers.Polymer chemistry · 2019Article
- Supramolecular Polymeric Assemblies for the Selective Depletion of Abundant Acidic Proteins in Serum.ACS applied materials & interfaces · 2018Article
- Molecular Features Influencing the Release of Peptides from Amphiphilic Polymeric Reverse Micelles.Langmuir : the ACS journal of surfaces and colloids · 2018Article
- Improved mass spectrometric detection of acidic peptides by variations in the functional group pKThe Analyst · 2018Article
- Self-assembly of random co-polymers for selective binding and detection of peptides.Polymer chemistry · 2018Article
- Supramolecular Assemblies for Transporting Proteins Across an Immiscible Solvent Interface.Journal of the American Chemical Society · 2018Article
- Altering the Peptide Binding Selectivity of Polymeric Reverse Micelle Assemblies via Metal Ion Loading.Langmuir : the ACS journal of surfaces and colloids · 2017Article
- Polymer-mediated ternary supramolecular interactions for sensitive detection of peptides.The Analyst · 2016Article
- Follicular fluid biomarkers for human in vitro fertilization outcome: Proof of principle.Proteome science · 2016Article
- Zwitterionic Amphiphilic Homopolymer Assemblies.Polymer chemistry · 2015Article
- Protein-triggered supramolecular disassembly: insights based on variations in ligand location in amphiphilic dendrons.Journal of the American Chemical Society · 2014Article
- Advances in Polymer and Polymeric Nanostructures for Protein Conjugation.European polymer journal · 2013Article
- Electrostatic control of peptide side-chain reactivity using amphiphilic homopolymer-based supramolecular assemblies.Journal of the American Chemical Society · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Reverse-micelle forming amphiphilic homopolymers with carboxylic acid and quaternary amine substituents are used to selectively enrich biomarker peptides and protein fragments from human serum prior to matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. After depletion of human serum albumin (HSA) and immunoglobulin G (IgG), low abundance peptide biomarkers can be selectively enriched and detected by MALDI-MS at clinically relevant concentrations by using the appropriate homopolymer(s) and extraction pH value(s). Three breast cancer peptide biomarkers, bradykinin, C4a, and ITIH(4), were chosen to test this new approach, and detection limits of 0.5 ng mL(-1), 0.08 ng mL(-1), and 0.2 ng mL(-1), respectively, were obtained. In addition, the amphiphilic homopolymers were used to detect prostate specific antigen (PSA) at concentrations as low as 0.5 ng mL(-1) by targeting a surrogate peptide fragment of this protein biomarker. Selective enrichment and sensitive MS detection of low abundance peptide/protein biomarkers by these polymeric reverse micelles should be a sensitive and straightforward approach for biomarker screening in human serum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.