ArticleLab on a chip2012
A silicone-based stretchable micropost array membrane for monitoring live-cell subcellular cytoskeletal response.
Article in Lab on a chip, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 105 citations in OpenAlex.
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- Metabolic-Glycoengineering-Enabled Molecularly Specific Acoustic Tweezing Cytometry for Targeted Mechanical Stimulation of Cell Surface Sialoglycans.Angewandte Chemie (International ed. in English) · 2024Article
- Development of a Pneumatic-Driven Fiber-Shaped Robot Scaffold for Use as a Complex 3D Dynamic Culture System.Biomimetics (Basel, Switzerland) · 2023Article
- Microdevice-based mechanical compression on living cells.iScience · 2022Review
- Biophysical Approaches for Applying and Measuring Biological Forces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Article
- Integrating mechanical sensor readouts into organ-on-a-chip platforms.Frontiers in bioengineering and biotechnology · 2022Review
- Revisiting tissue tensegrity: Biomaterial-based approaches to measure forces across length scales.APL bioengineering · 2021Review
- Microfluidic models of the human circulatory system: versatile platforms for exploring mechanobiology and disease modeling.Biophysical reviews · 2021Review
- High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology.Chemical reviews · 2021Review
- A microfluidics-based stem cell model of early post-implantation human development.Nature protocols · 2021Article
- Studying the Mechanobiology of Aortic Endothelial Cells Under Cyclic Stretch Using a Modular 3D Printed System.Frontiers in bioengineering and biotechnology · 2021Article
- (De)form and Function: Measuring Cellular Forces with Deformable Materials and Deformable Structures.Advanced healthcare materials · 2020Review
- Engineering Biomaterials and Approaches for Mechanical Stretching of Cells in Three Dimensions.Frontiers in bioengineering and biotechnology · 2020Review
- Probing Single-Cell Mechanical Allostasis Using Ultrasound Tweezers.Cellular and molecular bioengineering · 2019Article
- Cyclic Stretching of Fibrotic Microtissue Array for Evaluation of Anti-Fibrosis Drugs.Cellular and molecular bioengineering · 2019Article
- A Toolbox for Organelle Mechanobiology Research-Current Needs and Challenges.Micromachines · 2019Article
- Deformation Microscopy for Dynamic Intracellular and Intranuclear Mapping of Mechanics with High Spatiotemporal Resolution.Cell reports · 2019Article
- Mechanical response of an epithelial island subject to uniaxial stretch on a hybrid silicone substrate.Cellular and molecular bioengineering · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
External forces are increasingly recognized as major regulators of cellular structure and function, yet the underlying mechanism by which cells sense forces and transduce them into intracellular biochemical signals and behavioral responses ('mechanotransduction') is largely undetermined. To aid in the mechanistic study of mechanotransduction, herein we devised a cell stretching device that allowed for quantitative control and real-time measurement of mechanical stimuli and cellular biomechanical responses. Our strategy involved a microfabricated array of silicone elastomeric microposts integrated onto a stretchable elastomeric membrane. Using a computer-controlled vacuum, this micropost array membrane (mPAM) was activated to apply equibiaxial cell stretching forces to adherent cells attached to the microposts. Using the mPAM, we studied the live-cell subcellular dynamic responses of contractile forces in vascular smooth muscle cells (VSMCs) to a sustained static equibiaxial cell stretch. Our data showed that in response to a sustained cell stretch, VSMCs regulated their cytoskeletal (CSK) contractility in a biphasic manner: they first acutely enhanced their contraction to resist rapid cell deformation ('stiffening') before they allowed slow adaptive inelastic CSK reorganization to release their contractility ('softening'). The contractile response across entire single VSMCs was spatially inhomogeneous and force-dependent. Our mPAM device and live-cell subcellular contractile measurements will help elucidate the mechanotransductive system in VSMCs and thus contribute to our understanding of pressure-induced vascular disease processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.