ArticleBMC microbiology2011
Reliable identification at the species level of Brucella isolates with MALDI-TOF-MS.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- A MALDI-ToF mass spectrometry database for identification and classification of highly pathogenic bacteria.Scientific data · 2025Article
- Article
- Microbiological Laboratory Diagnosis of Human Brucellosis: An Overview.Pathogens (Basel, Switzerland) · 2021Review
- Whole-Genome Sequencing for Tracing the Genetic Diversity ofPathogens (Basel, Switzerland) · 2021Article
- Protein Biomarker Identification for the Discrimination ofFrontiers in microbiology · 2021Article
- Canine Brucellosis: An Update.Frontiers in veterinary science · 2021Review
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based identification of security-sensitive bacteria: Considerations for Canadian Bruker users.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2020Article
- Proteomics ofMicroorganisms · 2020Article
- Brucellosis - laboratory workers' nightmare come true: A case study.African journal of laboratory medicine · 2020Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Whole Genome Sequencing for Tracing Geographical Origin of Imported Cases of Human Brucellosis in Sweden.Microorganisms · 2019Article
- Comparison of standard and on-plate extraction protocols for identification of mastitis-causing bacteria by MALDI-TOF MS.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Answer to March 2019 Photo Quiz.Journal of clinical microbiology · 2019Article
- Evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying VIM- and SPM-type metallo-β-lactamase-producingInfection and drug resistance · 2019Article
- The Brief Case: Misidentification of Brucella melitensis as Ochrobactrum anthropi by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).Journal of clinical microbiology · 2018Article
- Misidentification of Risk Group 3/Security Sensitive Biological Agents by MALDI-TOF MS in Canada: November 2015-October 2017.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2018Article
- Article
- A novel real-time PCR assay for specific detection of Brucella melitensis.BMC infectious diseases · 2017Article
- Verification of a Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Method for Diagnostic Identification of High-Consequence Bacterial Pathogens.Journal of clinical microbiology · 2016Article
- A Simple and Safe Protocol for Preparing Brucella Samples for Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Analysis.Journal of clinical microbiology · 2016Article
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12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe genus Brucella contains highly infectious species that are classified as biological threat agents. The timely detection and identification of the microorganism involved is essential for an effective response not only to biological warfare attacks but also to natural outbreaks. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a rapid method for the analysis of biological samples. The advantages of this method, compared to conventional techniques, are rapidity, cost-effectiveness, accuracy and suitability for the high-throughput identification of bacteria. Discrepancies between taxonomy and genetic relatedness on the species and biovar level complicate the development of detection and identification assays.
resultsIn this study, the accurate identification of Brucella species using MALDI-TOF-MS was achieved by constructing a Brucella reference library based on multilocus variable-number tandem repeat analysis (MLVA) data. By comparing MS-spectra from Brucella species against a custom-made MALDI-TOF-MS reference library, MALDI-TOF-MS could be used as a rapid identification method for Brucella species. In this way, 99.3% of the 152 isolates tested were identified at the species level, and B. suis biovar 1 and 2 were identified at the level of their biovar. This result demonstrates that for Brucella, even minimal genomic differences between these serovars translate to specific proteomic differences.
conclusionsMALDI-TOF-MS can be developed into a fast and reliable identification method for genetically highly related species when potential taxonomic and genetic inconsistencies are taken into consideration during the generation of the reference library.
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